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タイトル: | Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts |
著者: | Thagun, Chonprakun Horii, Yoko Mori, Maai Fujita, Seiya Ohtani, Misato Tsuchiya, Kousuke Kodama, Yutaka Odahara, Masaki Numata, Keiji https://orcid.org/0000-0003-2199-7420 (unconfirmed) |
著者名の別形: | タグン, チョンパラカン 堀井, 陽子 藤田, 聖矢 大谷, 美沙都 土屋, 康佑 児玉, 豊 小田原, 真樹 沼田, 圭司 |
キーワード: | Plants Peptides and proteins Genetics Fluorescence Molecules DNA siRNA peptide spraying plant cell chloroplast nanocarrier |
発行日: | 22-Mar-2022 |
出版者: | American Chemical Society (ACS) |
誌名: | ACS Nano |
巻: | 16 |
号: | 3 |
開始ページ: | 3506 |
終了ページ: | 3521 |
抄録: | Genetic engineering of economically important traits in plants is an effective way to improve global welfare. However, introducing foreign DNA molecules into plant genomes to create genetically engineered plants not only requires a lengthy testing period and high developmental costs but also is not well-accepted by the public due to safety concerns about its effects on human and animal health and the environment. Here, we present a high-throughput nucleic acids delivery platform for plants using peptide nanocarriers applied to the leaf surface by spraying. The translocation of sub-micrometer-scale nucleic acid/peptide complexes upon spraying varied depending on the physicochemical characteristics of the peptides and was controlled by a stomata-dependent-uptake mechanism in plant cells. We observed efficient delivery of DNA molecules into plants using cell-penetrating peptide (CPP)-based foliar spraying. Moreover, using foliar spraying, we successfully performed gene silencing by introducing small interfering RNA molecules in plant nuclei via siRNA-CPP complexes and, more importantly, in chloroplasts via our CPP/chloroplast-targeting peptide-mediated delivery system. This technology enables effective nontransgenic engineering of economically important plant traits in agricultural systems. |
記述: | 【研究成果】スプレーで植物を改変 --簡便な非遺伝子組換え植物改変法の開発--. 京都大学プレスリリース. 2022-02-24. |
著作権等: | © 2022 The Authors. Published by American Chemical Society This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
URI: | http://hdl.handle.net/2433/268965 |
DOI(出版社版): | 10.1021/acsnano.1c07723 |
PubMed ID: | 35195009 |
関連リンク: | https://www.t.kyoto-u.ac.jp/ja/news/topics/research/20220223-2 |
出現コレクション: | 学術雑誌掲載論文等 |
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