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タイトル: | Microwell bag culture for large-scale production of homogeneous islet-like clusters |
著者: | Suenaga, Ryo Konagaya, Shuhei Yamaura, Junji Ito, Ryo Tanaka, Satoshi Ishizaki, Yoichi Toyoda, Taro https://orcid.org/0000-0002-2948-0525 (unconfirmed) |
著者名の別形: | 末永, 亮 小長谷, 周平 伊藤, 亮 豊田, 太郎 |
キーワード: | Biomaterials Regenerative medicine Stem-cell biotechnology |
発行日: | 2022 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 12 |
論文番号: | 5221 |
抄録: | Pluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 10⁵–10⁶ islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to be overcome for its clinical use. In this study, we aimed to develop a novel bag culture method for the production of uniform cell clusters on a large scale (10⁵–10⁶ clusters). We prepared small-scale culture bags (< 10⁵ clusters) with microwells at the bottom and optimized the conditions for producing uniform-sized clusters in the bag using undifferentiated induced pluripotent stem cells (iPSCs). Subsequently, we verified the suitability of the bag culture method using iPSC-derived pancreatic islet cells (iPICs) and successfully demonstrate the production of 6.5 × 10⁵ uniform iPIC clusters using a large-scale bag. In addition, we simplified the pre- and post-process of the culture—a degassing process before cell seeding and a cluster harvesting process. In conclusion, compared with conventional methods, the cluster production method using bags exhibits improved scalability, sterility, and operability for both clinical and research use. |
記述: | 均一な膵島様細胞凝集塊の大規模生産のためのマイクロウェルバッグ培養. 京都大学プレスリリース. 2022-04-15. Microwell bag culture for large-scale production of homogeneous islet-like cell clusters. 京都大学プレスリリース. 2022-05-02. |
著作権等: | © The Author(s) 2022 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/269449 |
DOI(出版社版): | 10.1038/s41598-022-09124-w |
PubMed ID: | 35338209 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/220415-100002.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/220502-090000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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