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タイトル: | Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technology |
著者: | Shimode, Sayumi Sakuma, Tetsushi Yamamoto, Takashi Miyazawa, Takayuki |
著者名の別形: | 下出, 紗弓 佐久間, 哲史 山本, 卓 宮沢, 孝幸 |
キーワード: | Live attenuated vaccines Microbiology Molecular engineering Retrovirus Vaccines Virology |
発行日: | 2022 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 12 |
論文番号: | 6641 |
抄録: | Endogenous retroviruses (ERVs) are retroviral sequences present in the host genomes. Although most ERVs are inactivated, some are produced as replication-competent viruses from host cells. We previously reported that several live-attenuated vaccines for companion animals prepared using the Crandell-Rees feline kidney (CRFK) cell line were contaminated with a replication-competent feline ERV termed RD-114 virus. We also found that the infectious RD-114 virus can be generated by recombination between multiple RD-114 virus-related proviruses (RDRSs) in CRFK cells. In this study, we knocked out RDRS env genes using the genome-editing tool TAL Effector Nuclease (TALEN) to reduce the risk of contamination by infectious ERVs in vaccine products. As a result, we succeeded in establishing RDRS knockout CRFK cells (RDKO_CRFK cells) that do not produce infectious RD-114 virus. The growth kinetics of feline herpesvirus type 1, calicivirus, and panleukopenia virus in RDKO_CRFK cells differed from those in parental cells, but all of them showed high titers exceeding 10⁷ TCID₅₀/mL. Infectious RD-114 virus was undetectable in the viral stocks propagated in RDKO_CRFK cells. This study suggested that RDRS env gene-knockout CRFK cells will be useful as a cell line for the manufacture of live-attenuated vaccines or biological substances with no risk of contamination with infectious ERV. |
記述: | ゲノム編集による内在性レトロウイルス排除 --より安全なワクチン製造を目指して--. 京都大学プレスリリース. 2022-04-28. |
著作権等: | © The Author(s) 2022 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/269539 |
DOI(出版社版): | 10.1038/s41598-022-10497-1 |
PubMed ID: | 35477976 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-04-28 |
出現コレクション: | 学術雑誌掲載論文等 |
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