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タイトル: | Multi-omics approach reveals post-transcriptionally regulated genes are essential for human pluripotent stem cells |
著者: | Iwasaki, Mio https://orcid.org/0000-0003-2085-732X (unconfirmed) Kawahara, Yuka Okubo, Chikako Yamakawa, Tatsuya https://orcid.org/0009-0004-3986-8613 (unconfirmed) Nakamura, Michiko Tabata, Tsuyoshi Nishi, Yohei Narita, Megumi Ohta, Akira Saito, Hirohide Yamamoto, Takuya https://orcid.org/0000-0002-0022-3947 (unconfirmed) Nakagawa, Masato https://orcid.org/0000-0003-3067-7322 (unconfirmed) Yamanaka, Shinya Takahashi, Kazutoshi |
著者名の別形: | 岩崎, 未央 川原, 優香 大久保, 周子 山川, 達也 中村, 美千子 田畑, 剛 西, 洋平 成田, 恵 太田, 章 齊藤, 博英 山本, 拓也 中川, 誠人 山中, 伸弥 高橋, 和利 |
キーワード: | Biological sciences Stem cells research Omics |
発行日: | May-2022 |
出版者: | Elsevier BV |
誌名: | iScience |
巻: | 25 |
号: | 5 |
論文番号: | 104289 |
抄録: | The effects of transcription factors on the maintenance and differentiation of human-induced or embryonic pluripotent stem cells (iPSCs/ESCs) have been well studied. However, the importance of posttranscriptional regulatory mechanisms, which cause the quantitative dissociation of mRNA and protein expression, has not been explored in detail. Here, by combining transcriptome and proteome profiling, we identified 228 posttranscriptionally regulated genes with strict upregulation of the protein level in iPSCs/ESCs. Among them, we found 84 genes were vital for the survival of iPSCs and HDFs, including 20 genes that were specifically necessary for iPSC survival. These 20 proteins were upregulated only in iPSCs/ESCs and not in differentiated cells derived from the three germ layers. Although there are still unknown mechanisms that downregulate protein levels in HDFs, these results reveal that posttranscriptionally regulated genes have a crucial role in iPSC survival. |
記述: | ヒトのiPS細胞やES細胞の生存に必要な遺伝子の発見 --これまで見過ごされていた、タンパク質レベルで量が制御されている遺伝子群の制御機構解明に向けて--. 京都大学プレスリリース. 2022-05-13. Discovery of genes required for survival of human iPS and ES cells. 京都大学プレスリリース. 2022-05-16. |
著作権等: | © 2022 The Authors. This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
URI: | http://hdl.handle.net/2433/270024 |
DOI(出版社版): | 10.1016/j.isci.2022.104289 |
PubMed ID: | 35573189 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/220513-140000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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