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このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| j.xpro.2021.100682.pdf | 3.78 MB | Adobe PDF | 見る/開く |
| タイトル: | A protocol for analyzing the protein terminome of human cancer cell line culture supernatants |
| 著者: | Tsumagari, Kazuya Chang, Chih-Hsiang Ishihama, Yasushi    https://orcid.org/0000-0001-7714-203X (unconfirmed) |
| 著者名の別形: | 津曲, 和哉 石濱, 泰 |
| キーワード: | Cell Membrane Protein Biochemistry Proteomics Mass Spectrometry Systems biology |
| 発行日: | 17-Sep-2021 |
| 出版者: | Elsevier BV |
| 誌名: | STAR Protocols |
| 巻: | 2 |
| 号: | 3 |
| 論文番号: | 100682 |
| 抄録: | Characterization of protein termini is essential for understanding how the proteome is generated through biological processes such as post-translational proteolytic events. Here, we introduce a practical protocol for terminomics to achieve simple and sensitive N- and C-terminal peptide enrichment. We apply it to the terminome analysis of culture supernatants of a human cancer cell line for the purpose of identifying ectodomain shedding substrate cleavage sites with 10 μg protein per sample. |
| 著作権等: | © 2021 The Author(s). This is an open access article under the Creative Commons Attribution 4.0 International license. |
| URI: | http://hdl.handle.net/2433/274018 |
| DOI(出版社版): | 10.1016/j.xpro.2021.100682 |
| PubMed ID: | 34377995 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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