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タイトル: | A light-induced small G-protein gem limits the circadian clock phase-shift magnitude by inhibiting voltage-dependent calcium channels |
著者: | Matsuo, Masahiro Seo, Kazuyuki Taruno, Akiyuki Mizoro, Yasutaka Yamaguchi, Yoshiaki https://orcid.org/0000-0003-4126-542X (unconfirmed) Doi, Masao https://orcid.org/0000-0001-6264-9217 (unconfirmed) Nakao, Rhyuta Kori, Hiroshi Abe, Takaya Ohmori, Harunori Tominaga, Keiko Okamura, Hitoshi |
著者名の別形: | 松尾, 雅博 瀨尾, 和志 樽野, 陽幸 溝曽路, 祥孝 山口, 賀章 土居, 雅夫 中尾, 龍太 郡, 宏 阿部, 高也 大森, 治紀 冨永, 恵子 岡村, 均 |
キーワード: | RGK Gem small G-protein suprachiasmatic nucleus circadian clock light-induced phase-shift voltage-dependent calcium channels |
発行日: | May-2022 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 39 |
号: | 8 |
論文番号: | 110844 |
抄録: | Calcium signaling is pivotal to the circadian clockwork in the suprachiasmatic nucleus (SCN), particularly in rhythm entrainment to environmental light-dark cycles. Here, we show that a small G-protein Gem, an endogenous inhibitor of high-voltage-activated voltage-dependent calcium channels (VDCCs), is rapidly induced by light in SCN neurons via the calcium (Ca²⁺)-mediated CREB/CRE transcriptional pathway. Gem attenuates light-induced calcium signaling through its interaction with VDCCs. The phase-shift magnitude of locomotor activity rhythms by light, at night, increases in Gem-deficient (Gem⁻/⁻) mice. Similarly, in SCN slices from Gem⁻/⁻ mice, depolarizing stimuli induce larger phase shifts of clock gene transcription rhythms that are normalized by the application of an L-type VDCC blocker, nifedipine. Voltage-clamp recordings from SCN neurons reveal that Ca²⁺ currents through L-type channels increase in Gem⁻/⁻ mice. Our findings suggest that transcriptionally activated Gem feeds back to suppress excessive light-evoked L-type VDCC activation, adjusting the light-induced phase-shift magnitude to an appropriate level in mammals. |
記述: | 光誘導物質による新しい体内時計の同調制御機構 --光により誘導される神経細胞の活動抑制物質の発見--. 京都大学プレスリリース. 2022-05-26. |
著作権等: | © 2022 The Authors. This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
URI: | http://hdl.handle.net/2433/274091 |
DOI(出版社版): | 10.1016/j.celrep.2022.110844 |
PubMed ID: | 35613591 |
出現コレクション: | 学術雑誌掲載論文等 |
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