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タイトル: | Revisiting the concept of peptide bond planarity in an iron-sulfur protein by neutron structure analysis |
著者: | Hanazono, Yuya Hirano, Yu Takeda, Kazuki https://orcid.org/0000-0002-4094-6816 (unconfirmed) Kusaka, Katsuhiro Tamada, Taro Miki, Kunio |
著者名の別形: | 花園, 祐矢 平野, 優 竹田, 一旗 日下, 勝弘 玉田, 太郎 三木, 邦夫 |
発行日: | May-2022 |
出版者: | American Association for the Advancement of Science (AAAS) |
誌名: | Science Advances |
巻: | 8 |
号: | 20 |
論文番号: | eabn2276 |
抄録: | The planarity of the peptide bond is important for the stability and structure formation of proteins. However, substantial distortion of peptide bonds has been reported in several high-resolution structures and computational analyses. To investigate the peptide bond planarity, including hydrogen atoms, we report a 1.2-Å resolution neutron structure of the oxidized form of high-potential iron-sulfur protein. This high-resolution neutron structure shows that the nucleus positions of the amide protons deviate from the peptide plane and shift toward the acceptors. The planarity of the H─N─C═O plane depends strongly on the pyramidalization of the nitrogen atom. Moreover, the orientation of the amide proton of Cys⁷⁵ is different in the reduced and oxidized states, possibly because of the electron storage capacity of the iron-sulfur cluster. |
記述: | タンパク質の立体構造解析に新たなモデルを提唱 --より正確な立体構造の観測や予測を実現! 生命科学研究の進展に寄与--. 京都大学プレスリリース. 2022-05-27. |
著作権等: | Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/274106 |
DOI(出版社版): | 10.1126/sciadv.abn2276 |
PubMed ID: | 35594350 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-05-27-3 |
出現コレクション: | 学術雑誌掲載論文等 |
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