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j.bbrc.2021.12.066.pdf | 3.08 MB | Adobe PDF | 見る/開く |
タイトル: | Pluripotency state of mouse ES cells determines their contribution to self-organized layer formation by mesh closure on microstructured adhesion-limiting substrates |
著者: | Ando, Yuta https://orcid.org/0000-0002-9096-0640 (unconfirmed) Okeyo, Kennedy Omondi Adachi, Taiji https://orcid.org/0000-0001-5280-4156 (unconfirmed) |
著者名の別形: | 安藤, 悠太 安達, 泰治 |
キーワード: | Embryonic stem cells Pluripotency Naïve state Ground state Layer formation Mesh substrates |
発行日: | 29-Jan-2022 |
出版者: | Elsevier BV |
誌名: | Biochemical and Biophysical Research Communications |
巻: | 590 |
開始ページ: | 97 |
終了ページ: | 102 |
抄録: | Assembly of pluripotent stem cells to initiate self-organized tissue formation on engineered scaffolds is an important process in stem cell engineering. Pluripotent stem cells are known to exist in diverse pluripotency states, with heterogeneous subpopulations exhibiting differential gene expression levels, but how such diverse pluripotency states orchestrate tissue formation is still an unrevealed question. In this study, using microstructured adhesion-limiting substrates, we aimed to clarify the contribution to self-organized layer formation by mouse embryonic stem cells in different pluripotency states: ground and naïve state. We found that while ground state cells as well as sorted REX1-high expression cells formed discontinuous cell layers with limited lateral spread, naïve state cells could successfully self-organize to form a continuous layer by progressive mesh closure within 3 days. Using sequential immunofluorescence microscopy to examine the mesh closure process, we found that KRT8+ cells were particularly localized around unfilled holes, occasionally bridging the holes in a manner suggestive of their role in the closure process. These results highlight that compared with ground state cells, naïve state cells possess a higher capability to contribute to self-organized layer formation by mesh closure. Thus, this study provides insights with implications for the application of stem cells in scaffold-based tissue engineering. |
著作権等: | © 2021 The Authors. Published by Elsevier Inc. This is an open access article under the Creative Commons Attribution 4.0 International license. |
URI: | http://hdl.handle.net/2433/274518 |
DOI(出版社版): | 10.1016/j.bbrc.2021.12.066 |
PubMed ID: | 34973536 |
出現コレクション: | 学術雑誌掲載論文等 |
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