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タイトル: | Discovery of a phase-separating small molecule that selectively sequesters tubulin in cells |
著者: | Ado, Genyir Noda, Naotaka Vu, Hue T. Perron, Amelie Mahapatra, Amarjyoti D. Arista, Karla Pineda Yoshimura, Hideaki Packwood, Daniel M. Ishidate, Fumiyoshi Sato, Shin-ichi Ozawa, Takeaki Uesugi, Motonari https://orcid.org/0000-0002-8515-445X (unconfirmed) |
著者名の別形: | 野田, 尚孝 石館, 文善 佐藤, 慎一 上杉, 志成 |
発行日: | 20-Apr-2022 |
出版者: | Royal Society of Chemistry (RSC) |
誌名: | Chemical Science |
巻: | 13 |
号: | 19 |
開始ページ: | 5760 |
終了ページ: | 5766 |
抄録: | Phase-separated membraneless organelles or biomolecular condensates play diverse functions in cells, however recapturing their characteristics using small organic molecules has been a challenge. In the present study, cell-lysate-based screening of 843 self-assembling small molecules led to the discovery of a simple organic molecule, named huezole, that forms liquid droplets to selectively sequester tubulin. Remarkably, this small molecule enters cultured human cells and prevents cell mitosis by forming tubulin-concentrating condensates in cells. The present study demonstrates the feasibility of producing a synthetic condensate out of non-peptidic small molecules for exogenous control of cellular processes. The modular structure of huezole provides a framework for designing a class of organelle-emulating small molecules. |
著作権等: | © The Royal Society of Chemistry 2022 This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
URI: | http://hdl.handle.net/2433/274623 |
DOI(出版社版): | 10.1039/d1sc07151c |
PubMed ID: | 35694339 |
出現コレクション: | 学術雑誌掲載論文等 |
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