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j.celrep.2021.109875.pdf | 4.38 MB | Adobe PDF | 見る/開く |
タイトル: | A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis |
著者: | Nakagawa, Toshinori Jörg, David J. Watanabe, Hitomi Mizuno, Seiya Han, Seungmin Ikeda, Tatsuro Omatsu, Yoshiki Nishimura, Keiko Fujita, Miyako Takahashi, Satoru Kondoh, Gen Simons, Benjamin D. Yoshida, Shosei Nagasawa, Takashi |
著者名の別形: | 中川, 俊徳 渡邊, 仁美 尾松, 芳樹 近藤, 玄 長澤, 丘司 |
キーワード: | spermatogenic stem cells stem cell heterogeneity lineage tracing Plvap Sox3 tissue homeostasis testis mice |
発行日: | Oct-2021 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 37 |
号: | 3 |
論文番号: | 109875 |
抄録: | In mouse testis, a heterogeneous population of undifferentiated spermatogonia (A[undiff]) harbors spermatogenic stem cell (SSC) potential. Although GFRα1⁺ Aundiff maintains the self-renewing pool in homeostasis, the functional basis of heterogeneity and the implications for their dynamics remain unresolved. Here, through quantitative lineage tracing of SSC subpopulations, we show that an ensemble of heterogeneous states of SSCs supports homeostatic, persistent spermatogenesis. Such heterogeneity is maintained robustly through stochastic interconversion of SSCs between a renewal-biased Plvap⁺/GFRα1⁺ state and a differentiation-primed Sox3⁺/GFRα1⁺ state. In this framework, stem cell commitment occurs not directly but gradually through entry into licensed but uncommitted states. Further, Plvap⁺/GFRα1⁺ cells divide slowly, in synchrony with the seminiferous epithelial cycle, while Sox3⁺/GFRα1⁺ cells divide much faster. Such differential cell-cycle dynamics reduces mitotic load, and thereby the potential to acquire harmful de novo mutations of the self-renewing pool, while keeping the SSC density high over the testicular open niche. |
著作権等: | © 2021 The Author(s). This is an open access article under the Creative Commons Attribution 4.0 International license. |
URI: | http://hdl.handle.net/2433/274694 |
DOI(出版社版): | 10.1016/j.celrep.2021.109875 |
PubMed ID: | 34686326 |
出現コレクション: | 学術雑誌掲載論文等 |
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