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タイトル: | Modeling cell apoptosis for simulating three-dimensional multicellular morphogenesis based on a reversible network reconnection framework |
著者: | Okuda, Satoru Inoue, Yasuhiro https://orcid.org/0000-0002-1968-8883 (unconfirmed) Eiraku, Mototsugu Adachi, Taiji https://orcid.org/0000-0001-5280-4156 (unconfirmed) Sasai, Yoshiki |
著者名の別形: | 井上, 康博 安達, 泰治 |
キーワード: | Multicellular morphogenesis Cell apoptosis Apoptotic force Three-dimensional vertex model Reversible network reconnection model Computational biomechanics |
発行日: | Aug-2016 |
出版者: | Springer Nature |
誌名: | Biomechanics and Modeling in Mechanobiology |
巻: | 15 |
号: | 4 |
開始ページ: | 805 |
終了ページ: | 816 |
抄録: | Morphogenesis in multicellular organisms is accompanied by apoptotic cell behaviors: cell shrinkage and cell disappearance. The mechanical effects of these behaviors are spatiotemporally regulated within multicellular dynamics to achieve proper tissue sizes and shapes in three-dimensional (3D) space. To analyze 3D multicellular dynamics, 3D vertex models have been suggested, in which a reversible network reconnection (RNR) model has successfully expressed 3D cell rearrangements during large deformations. To analyze the effects of apoptotic cell behaviors on 3D multicellular morphogenesis, we modeled cell apoptosis based on the RNR model framework. Cell shrinkage was modeled by the potential energy as a function of individual cell times during the apoptotic phase. Cell disappearance was modeled by merging neighboring polyhedrons at their boundary surface according to the topological rules of the RNR model. To establish that the apoptotic cell behaviors could be expressed as modeled, we simulated morphogenesis driven by cell apoptosis in two types of tissue topology: 3D monolayer cell sheet and 3D compacted cell aggregate. In both types of tissue topology, the numerical simulations successfully illustrated that cell aggregates gradually shrank because of successive cell apoptosis. During tissue shrinkage, the number of cells in aggregates decreased while maintaining individual cell size and shape. Moreover, in case of localizing apoptotic cells within a part of the 3D monolayer cell aggregate, the cell apoptosis caused the global tissue bending by pulling on surrounding cells. In case of localizing apoptotic cells on the surface of the 3D compacted cell aggregate, the cell apoptosis caused successive, directional cell rearrangements from the inside to the surface. Thus, the proposed model successfully provided a basis for expressing apoptotic cell behaviors during 3D multicellular morphogenesis based on an RNR model framework. |
著作権等: | This is a post-peer-review, pre-copyedit version of an article published in 'Biomechanics and Modeling in Mechanobiology'. The final authenticated version is available online at: https://doi.org/10.1007/s10237-015-0724-7. The full-text file will be made open to the public on 11 September 2016 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/274887 |
DOI(出版社版): | 10.1007/s10237-015-0724-7 |
PubMed ID: | 26361766 |
出現コレクション: | 学術雑誌掲載論文等 |
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