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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| j.xpro.2022.101421.pdf | 4.44 MB | Adobe PDF | 見る/開く |
| タイトル: | In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice |
| 著者: | Sakamoto, Masayuki    https://orcid.org/0000-0001-6458-113X (unconfirmed)Ota, Keisuke Kondo, Yayoi Okamura, Michiko Fujii, Hajime Bito, Haruhiko |
| 著者名の別形: | 坂本, 雅行 |
| キーワード: | Developmental biology Microscopy Model Organisms Neuroscience |
| 発行日: | 17-Jun-2022 |
| 出版者: | Elsevier BV |
| 誌名: | STAR Protocols |
| 巻: | 3 |
| 号: | 2 |
| 論文番号: | 101421 |
| 抄録: | We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice. |
| 著作権等: | © 2022 The Author(s). This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
| URI: | http://hdl.handle.net/2433/275530 |
| DOI(出版社版): | 10.1016/j.xpro.2022.101421 |
| PubMed ID: | 35693213 |
| 出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス
