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このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| j.xpro.2021.100921.pdf | 2.94 MB | Adobe PDF | 見る/開く |
| タイトル: | Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction |
| 著者: | Io, Shingo Iemura, Yoshiki Takashima, Yasuhiro    https://orcid.org/0000-0003-0126-7260 (unconfirmed) |
| 著者名の別形: | 伊尾, 紳吾 家村, 宜樹 髙島, 康弘 |
| キーワード: | Cell Biology Cell culture Cell Differentiation Developmental biology Flow Cytometry/Mass Cytometry Molecular Biology Stem Cells |
| 発行日: | 17-Dec-2021 |
| 出版者: | Elsevier BV |
| 誌名: | STAR Protocols |
| 巻: | 2 |
| 号: | 4 |
| 論文番号: | 100921 |
| 抄録: | Human trophoblasts arise from the morula as trophectoderm, which differentiates into cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast after implantation. Here, we present a robust step-by-step protocol to induce trophectoderm (TE) from naive human pluripotent stem cells (PSCs) corresponding to pre-implantation epiblast. Our culture system (TE induction and ACE condition) mimics the entire trophoblast development including the molecular events. For complete details on the use and execution of this protocol, please refer to Io et al. (2021). |
| 著作権等: | © 2021 The Authors. This is an open access article under the Creative Commons Attribution 4.0 International license. |
| URI: | http://hdl.handle.net/2433/275678 |
| DOI(出版社版): | 10.1016/j.xpro.2021.100921 |
| PubMed ID: | 34761233 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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