Insights into the mechanism of diurnal variations in methane emission from the stem surfaces of Alnus japonica

Abstract

Recent studies have suggested that in certain environments, tree stems emit methane (CH₄). This study explored the mechanism of CH₄ emission from the stem surfaces of Alnus japonica in a riparian wetland. Stem CH₄ emission rates and sap flux were monitored year-round, and fine-root anatomy was investigated. CH₄ emission rates were estimated using a closed-chamber method. Sap flux was measured using Granier-type thermal dissipation probes. Root anatomy was studied using both optical and cryo-scanning electron microscopy. CH₄ emissions during the leafy season exhibited a diurnally changing component superimposed upon an underlying continuum in which the diurnal variation was in phase with sap flux. We propose a model in which stem CH₄ emission involves at least two processes: a sap flux-dependent component responsible for the diurnal changes, and a sap flux-independent component responsible for the background continuum. The contribution ratios of the two processes are season-dependent. The background continuum possibly resulted from the diffusive transport of gaseous CH₄ from the roots to the upper trunk. Root anatomy analysis indicated that the intercellular space of the cortex and empty xylem cells in fine roots could serve as a passageway for transport of gaseous CH₄.