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ファイル | 記述 | サイズ | フォーマット | |
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j.crmeth.2021.100155.pdf | 3.74 MB | Adobe PDF | 見る/開く |
タイトル: | A stress-reduced passaging technique improves the viability of human pluripotent cells |
著者: | Takahashi, Kazutoshi Okubo, Chikako Nakamura, Michiko Iwasaki, Mio https://orcid.org/0000-0003-2085-732X (unconfirmed) Kawahara, Yuka Tabata, Tsuyoshi Miyamoto, Yousuke Woltjen, Knut https://orcid.org/0000-0003-2293-1183 (unconfirmed) Yamanaka, Shinya |
著者名の別形: | 高橋, 和利 大久保, 周子 中村, 美千子 岩崎, 未央 川原, 優香 田畑, 剛 宮本, 洋佑 山中, 伸弥 |
キーワード: | human pluripotent stem cell xeno-free culture DNA damage passage viability reproducibility |
発行日: | 28-Feb-2022 |
出版者: | Elsevier BV |
誌名: | Cell Reports Methods |
巻: | 2 |
号: | 2 |
論文番号: | 100155 |
抄録: | Xeno-free culture systems have expanded the clinical and industrial application of human pluripotent stem cells (PSCs). However, reproducibility issues, often arising from variability during passaging steps, remain. Here, we describe an improved method for the subculture of human PSCs. The revised method significantly enhances the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications such as gene editing and directed differentiation. Furthermore, the method does not alter PSC characteristics after long-term culture and attenuates the growth advantage of abnormal subpopulations. This robust passaging method minimizes experimental error and reduces the rate of PSCs failing quality control of human PSC research and application. |
著作権等: | © 2021 The Author(s). This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
URI: | http://hdl.handle.net/2433/275832 |
DOI(出版社版): | 10.1016/j.crmeth.2021.100155 |
PubMed ID: | 35474962 |
出現コレクション: | 学術雑誌掲載論文等 |
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