ダウンロード数: 96
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.celrep.2022.111323.pdf | 3.12 MB | Adobe PDF | 見る/開く |
タイトル: | Structural insights into the G protein selectivity revealed by the human EP3-Gi signaling complex |
著者: | Suno, Ryoji Sugita, Yukihiko ![]() ![]() ![]() Morimoto, Kazushi Takazaki, Hiroko Tsujimoto, Hirokazu Hirose, Mika Suno-Ikeda, Chiyo Nomura, Norimichi ![]() ![]() ![]() Hino, Tomoya Inoue, Asuka Iwasaki, Kenji Kato, Takayuki Iwata, So ![]() ![]() Kobayashi, Takuya |
著者名の別形: | 寿野, 良二 杉田, 征彦 森本, 和志 髙﨑, 寛子 辻本, 浩一 廣瀬, 未果 寿野, 千代 野村, 紀通 日野, 智也 井上, 飛鳥 岩崎, 憲治 加藤, 貴之 岩田, 想 清水(小林), 拓也 |
キーワード: | G protein-coupled receptor prostaglandin receptor cryoelectron microscopy single-particle analysis signal transduction G protein-coupling selectivity |
発行日: | Sep-2022 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 40 |
号: | 11 |
論文番号: | 111323 |
抄録: | Prostaglandin receptors have been implicated in a wide range of functions, including inflammation, immune response, reproduction, and cancer. Our group has previously determined the crystal structure of the active-like EP3 bound to its endogenous agonist, prostaglandin E₂. Here, we present the single-particle cryoelectron microscopy (cryo-EM) structure of the human EP3-Gi signaling complex at a resolution of 3.4 Å. The structure reveals the binding mode of Gi to EP3 and the structural changes induced in EP3 by Gi binding. In addition, we compare the structure of the EP3-Gi complex with other subtypes of prostaglandin receptors (EP2 and EP4) bound to Gs that have been previously reported and examine the differences in amino acid composition at the receptor-G protein interface. Mutational analysis reveals that the selectivity of the G protein depends on specific amino acid residues in the second intracellular loop and TM5. |
記述: | 熱、炎症などに関与するプロスタグランジン受容体EP3シグナリング複合体の可視化 --緑内障、高眼圧症治療薬の合理的設計に貢献--. 京都大学プレスリリース. 2022-09-15. |
著作権等: | © 2022 The Author(s). This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
URI: | http://hdl.handle.net/2433/276290 |
DOI(出版社版): | 10.1016/j.celrep.2022.111323 |
PubMed ID: | 36103815 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-09-15 |
出現コレクション: | 学術雑誌掲載論文等 |
![](/dspace/image/articlelinker.gif)
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス