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s41536-022-00241-8.pdf | 5.6 MB | Adobe PDF | 見る/開く |
タイトル: | Induction of functional xeno-free MSCs from human iPSCs via a neural crest cell lineage |
著者: | Kamiya, Daisuke Takenaka-Ninagawa, Nana Motoike, Souta Kajiya, Mikihito Akaboshi, Teppei Zhao, Chengzhu Shibata, Mitsuaki Senda, Sho Toyooka, Yayoi Sakurai, Hidetoshi https://orcid.org/0000-0002-5383-9366 (unconfirmed) Kurihara, Hidemi Ikeya, Makoto https://orcid.org/0000-0002-3930-8032 (unconfirmed) |
著者名の別形: | 上谷, 大介 竹中, 菜々 本池, 総太 加治屋, 幹人 赤星, 哲平 趙, 成珠 柴田, 光章 千田, 将 豊岡, やよい 櫻井, 英俊 栗原, 英見 池谷, 真 |
キーワード: | Mesenchymal stem cells Regeneration Stem-cell research |
発行日: | 2022 |
出版者: | Springer Nature |
誌名: | npj Regenerative Medicine |
巻: | 7 |
論文番号: | 47 |
抄録: | Mesenchymal stem/stromal cells (MSCs) are adult multipotent stem cells. Here, we induced MSCs from human induced pluripotent stem cells (iPSCs) via a neural crest cell (NCC) lineage under xeno-free conditions and evaluated their in vivo functions. We modified a previous MSC induction method to work under xeno-free conditions. Bovine serum albumin-containing NCC induction medium and fetal bovine serum-containing MSC induction medium were replaced with xeno-free medium. Through our optimized method, iPSCs differentiated into MSCs with high efficiency. To evaluate their in vivo activities, we transplanted the xeno-free-induced MSCs (XF-iMSCs) into mouse models for bone and skeletal muscle regeneration and confirmed their regenerative potency. These XF-iMSCs mainly promoted the regeneration of surrounding host cells, suggesting that they secrete soluble factors into affected regions. We also found that the peroxidasin and IGF2 secreted by the XF-iMSCs partially contributed to myotube differentiation. These results suggest that XF-iMSCs are important for future applications in regenerative medicine. |
記述: | iPS細胞から間葉系幹細胞の誘導方法を確立 --動物由来成分を含まず再生医療への利用に期待. 京都大学プレスリリース. 2022-09-15. A new method for inducing mesenchymal stem cells from iPS cells without using animal-derived components. 京都大学プレスリリース. 2022-09-27. |
著作権等: | © The Author(s) 2022 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/276320 |
DOI(出版社版): | 10.1038/s41536-022-00241-8 |
PubMed ID: | 36109564 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/220915-180000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/220927-150000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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