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タイトル: Revealing 3D structure of gluten in wheat dough by optical clearing imaging
著者: Ogawa, Takenobu  kyouindb  KAKEN_id
Matsumura, Yasuki  kyouindb  KAKEN_id
著者名の別形: 小川, 剛伸
松村, 康生
キーワード: Fluorescence imaging
Proteins
発行日: 2021
出版者: Springer Nature
誌名: Nature Communications
巻: 12
論文番号: 1708
抄録: Gluten, which makes up 85% of endosperm wheat protein, is considered a crucial quality determinant of wheat-based food products. During wheat dough manufacture, the molecular packing of gluten causes formation of large structures that exceed the millimetre scale. However, due to lack of imaging techniques for complex systems composed of giant macromolecules, the entire gluten structure remains unknown. Here, we develop an optical clearing reagent (termed SoROCS) that makes wheat-based products transparent. Combined with two-photon microscopy, we image the three-dimensional (3D) structure of gluten at the size in the millimetre scale and at submicron resolution. Further, we demonstrate how the 3D structure of gluten dramatically changes from a honeycomb-shaped network to sparse large clumps in wheat noodles, depending on the salt added during dough making, thereby reducing stress when compressing the noodle. Moreover, we show that SoROCS can be used for noodle imaging using confocal laser scanning microscopy.
著作権等: © The Author(s) 2021
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
URI: http://hdl.handle.net/2433/276521
DOI(出版社版): 10.1038/s41467-021-22019-0
PubMed ID: 33731714
出現コレクション:学術雑誌掲載論文等

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