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タイトル: | Rice apoplastic CBM1-interacting protein counters blast pathogen invasion by binding conserved carbohydrate binding module 1 motif of fungal proteins |
著者: | Takeda, Takumi Takahashi, Machiko Shimizu, Motoki Sugihara, Yu Yamashita, Tetsuro Saitoh, Hiromasa Fujisaki, Koki Ishikawa, Kazuya Utsushi, Hiroe Kanzaki, Eiko Sakamoto, Yuichi Abe, Akira Terauchi, Ryohei https://orcid.org/0000-0002-0095-4651 (unconfirmed) |
著者名の別形: | 竹田, 匠 高橋, 真智子 清水, 元樹 杉原, 優 山下, 哲郎 齋藤, 宏昌 藤崎, 恒喜 石川, 和也 宇津志, 博恵 神崎, 英子 坂本, 裕一 阿部, 陽 寺内, 良平 |
キーワード: | Rice Mannose Leaves Plant fungal pathogens Sodium phosphate Antifungals Cellulose Plant cell walls |
発行日: | Sep-2022 |
出版者: | Public Library of Science (PLoS) |
誌名: | PLOS Pathogens |
巻: | 18 |
号: | 9 |
論文番号: | e1010792 |
抄録: | When infecting plants, fungal pathogens secrete cell wall-degrading enzymes (CWDEs) that break down cellulose and hemicellulose, the primary components of plant cell walls. Some fungal CWDEs contain a unique domain, named the carbohydrate binding module (CBM), that facilitates their access to polysaccharides. However, little is known about how plants counteract pathogen degradation of their cell walls. Here, we show that the rice cysteine-rich repeat secretion protein OsRMC binds to and inhibits xylanase MoCel10A of the blast fungus pathogen Magnaporthe oryzae, interfering with its access to the rice cell wall and degradation of rice xylan. We found binding of OsRMC to various CBM1-containing enzymes, suggesting that it has a general role in inhibiting the action of CBM1. OsRMC is localized to the apoplast, and its expression is strongly induced in leaves infected with M. oryzae. Remarkably, knockdown and overexpression of OsRMC reduced and enhanced rice defense against M. oryzae, respectively, demonstrating that inhibition of CBM1-containing fungal enzymes by OsRMC is crucial for rice defense. We also identified additional CBM-interacting proteins (CBMIPs) from Arabidopsis thaliana and Setaria italica, indicating that a wide range of plants counteract pathogens through this mechanism. |
記述: | いもち病からイネを守る細胞外タンパク質 (CBMIP) の発見 --CBMIPはイネの細胞壁を守り、いもち病菌の感染を抑える--. 京都大学プレスリリース. 2022-09-30. |
著作権等: | © 2022 Takeda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/276529 |
DOI(出版社版): | 10.1371/journal.ppat.1010792 |
PubMed ID: | 36173975 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-09-30 |
出現コレクション: | 学術雑誌掲載論文等 |
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