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タイトル: | Stimulation of uncoupling protein 1 expression by β-alanine in brown adipocytes |
著者: | Hamaoka, Tsukasa Fu, Xiajie Tomonaga, Shozo https://orcid.org/0000-0003-0888-1407 (unconfirmed) Hashimoto, Osamu Murakami, Masaru Funaba, Masayuki https://orcid.org/0000-0002-4303-6682 (unconfirmed) |
著者名の別形: | 浜岡, 司 友永, 省三 舟場, 正幸 |
キーワード: | β-alanine brown adipocytes UCP1 carnosine CREB |
発行日: | 30-Sep-2022 |
出版者: | Elsevier BV |
誌名: | Archives of Biochemistry and Biophysics |
巻: | 727 |
論文番号: | 109341 |
抄録: | Carnosine, which is abundant in meat, is a dipeptide composed of β-alanine and histidine, known to afford various health benefits. It has been suggested that carnosine can elicit an anti-obesity effect via induction and activation of brown/beige adipocytes responsible for non-shivering thermogenesis. However, the relationship between carnosine and brown/beige adipocytes has not been comprehensively elucidated. We hypothesized that β-alanine directly modulates brown/beige adipogenesis and performed an in vitro assessment to test this hypothesis. HB2 brown preadipocytes were differentiated using insulin from day 0. Cells were treated with various concentrations of β-alanine (12.5–100 μM) during adipogenesis (days 0–8) and differentiation (days 8–10). Then, cells were further stimulated with or without forskolin, an activator of the cAMP-dependent protein kinase pathway, on day 8 or day 10 for 4 h before harvesting. We observed that HB2 cells expressed molecules related to the transport and signal transduction of β-alanine. Treatment with β-alanine during brown adipogenesis dose-dependently enhanced forskolin-induced Ucp1 expression; this was not observed in differentiated brown adipocytes. Consistent with these findings, treatment with β-alanine during days 0–8 increased phosphorylation levels of CREB in forskolin-treated HB2 cells. In addition, β-alanine treatment during brown adipogenesis increased the expression of Pparα, known to induce brown/beige adipogenesis, in a dose-dependent manner. These findings revealed that β-alanine could target HB2 adipogenic cells and enhance forskolin-induced Ucp1 expression during brown adipogenesis, possibly by accelerating phosphorylation and activation of CREB. Thus, β-alanine, a carnosine-constituting amino acid, might directly act on brown adipogenic cells to stimulate energy expenditure. |
著作権等: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ The full-text file will be made open to the public on 30 September 2023 in accordance with publisher's 'Terms and Conditions for Self-Archiving' This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/276741 |
DOI(出版社版): | 10.1016/j.abb.2022.109341 |
PubMed ID: | 35777522 |
出現コレクション: | 学術雑誌掲載論文等 |
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