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j.celrep.2022.111059.pdf | 4.13 MB | Adobe PDF | 見る/開く |
タイトル: | A guiding role of the Arabidopsis circadian clock in cell differentiation revealed by time-series single-cell RNA sequencing |
著者: | Torii, Kotaro Inoue, Keisuke Bekki, Keita Haraguchi, Kazuya https://orcid.org/0000-0002-2479-3135 (unconfirmed) Kubo, Minoru Kondo, Yuki Suzuki, Takamasa Kubota, Akane Uemoto, Kyohei Shimizu, Hanako Saito, Masato Fukuda, Hiroo Araki, Takashi https://orcid.org/0000-0002-3616-1493 (unconfirmed) Endo, Motomu |
著者名の別形: | 鳥井, 孝太郎 井上, 佳祐 別城, 啓太 原口, 和也 上本, 恭平 清水, 華子 荒木, 崇 遠藤, 求 |
キーワード: | circadian clock cell differentiation single-cell RNA sequencing |
発行日: | 12-Jul-2022 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 40 |
号: | 2 |
論文番号: | 111059 |
抄録: | Circadian rhythms and progression of cell differentiation are closely coupled in multicellular organisms. However, whether establishment of circadian rhythms regulates cell differentiation or vice versa has not been elucidated due to technical limitations. Here, we exploit high cell fate plasticity of plant cells to perform single-cell RNA sequencing during the entire process of cell differentiation. By analyzing reconstructed actual time series of the differentiation processes at single-cell resolution using a method we developed (PeakMatch), we find that the expression profile of clock genes is changed prior to cell differentiation, including induction of the clock gene LUX ARRYTHMO (LUX). ChIP sequencing analysis reveals that LUX induction in early differentiating cells directly targets genes involved in cell-cycle progression to regulate cell differentiation. Taken together, these results not only reveal a guiding role of the plant circadian clock in cell differentiation but also provide an approach for time-series analysis at single-cell resolution. |
著作権等: | © 2022 The Authors. This is an open access article under the CC BY license. |
URI: | http://hdl.handle.net/2433/276853 |
DOI(出版社版): | 10.1016/j.celrep.2022.111059 |
PubMed ID: | 35830805 |
出現コレクション: | 学術雑誌掲載論文等 |
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