|Title:||Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids|
|Author's alias:||兩坂, 誠|
|Journal title:||STAR Protocols|
|Abstract:||The ureteric bud (UB) is a kidney precursor tissue that repeats branching morphogenesis and gives rise to the collecting ducts (CDs) and lower urinary tract. Here, we describe protocols to generate iUB organoids from human iPSCs; iUB organoids repeat branching morphogenesis. We describe how to expand iUB-organoid-derived tip colonies and how to induce CD progenitors from iUB organoids. These organoids can be used to study CD development and potentially as a model of kidney and urinary tract diseases.|
|Rights:||© 2022 The Author(s).|
This is an open access article under the CC BY license.
|Appears in Collections:||Journal Articles|
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