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Title: Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
Authors: Ryosaka, Makoto
Mae, Shin-Ichi
Osafune, Kenji
Author's alias: 兩坂, 誠
前, 伸一
長船, 健二
Keywords: Stem Cells
Cell Differentiation
Issue Date: Sep-2022
Publisher: Elsevier BV
Journal title: STAR Protocols
Volume: 3
Issue: 3
Thesis number: 101484
Abstract: The ureteric bud (UB) is a kidney precursor tissue that repeats branching morphogenesis and gives rise to the collecting ducts (CDs) and lower urinary tract. Here, we describe protocols to generate iUB organoids from human iPSCs; iUB organoids repeat branching morphogenesis. We describe how to expand iUB-organoid-derived tip colonies and how to induce CD progenitors from iUB organoids. These organoids can be used to study CD development and potentially as a model of kidney and urinary tract diseases.
Rights: © 2022 The Author(s).
This is an open access article under the CC BY license.
DOI(Published Version): 10.1016/j.xpro.2022.101484
PubMed ID: 35769929
Appears in Collections:Journal Articles

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