ダウンロード数: 54
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
jacsau.1c00504.pdf | 7.5 MB | Adobe PDF | 見る/開く |
タイトル: | A Synthetic Multidomain Peptide That Drives a Macropinocytosis-Like Mechanism for Cytosolic Transport of Exogenous Proteins into Plants |
著者: | Miyamoto, Takaaki Toyooka, Kiminori Chuah, Jo-Ann Odahara, Masaki Higchi-Takeuchi, Mieko Goto, Yumi Motoda, Yoko Kigawa, Takanori Kodama, Yutaka Numata, Keiji https://orcid.org/0000-0003-2199-7420 (unconfirmed) |
著者名の別形: | 沼田, 圭司 |
キーワード: | multidomain peptide protein delivery macropinocytosis chemical biology plant biotechnology |
発行日: | 24-Jan-2022 |
出版者: | American Chemical Society (ACS) |
誌名: | JACS Au |
巻: | 2 |
号: | 1 |
開始ページ: | 223 |
終了ページ: | 233 |
抄録: | Direct delivery of proteins into plants represents a promising alternative to conventional gene delivery for probing and modulating cellular functions without the risk of random integration of transgenes into the host genome. This remains challenging, however, because of the lack of a protein delivery tool applicable to diverse plant species and the limited information about the entry mechanisms of exogenous proteins in plant cells. Here, we present the synthetic multidomain peptide (named dTat-Sar-EED4) for cytosolic protein delivery in various plant species via simple peptide-protein coincubation. dTat-Sar-EED4 enabled the cytosolic delivery of an active enzyme with up to ∼20-fold greater efficiency than previously described cell-penetrating peptides in several model plant systems. Our analyses using pharmacological inhibitors and transmission electron microscopy revealed that dTat-Sar-EED4 triggered a unique endocytic mechanism for cargo protein internalization. This endocytic mechanism shares several features with macropinocytosis, including the dependency of actin polymerization, sensitivity to phosphatidylinositol-3 kinase activity, and formation of membrane protrusions and large intracellular vesicles (>200 nm in diameter), even though macropinocytosis has not been identified to date in plants. Our study thus presents a robust molecular tool that can induce a unique cellular uptake mechanism for the efficient transport of bioactive proteins into plants. |
著作権等: | Copyright © 2022 The Authors. Published by American Chemical Society This is an open access article published under a Creative Commons Non-Commercial NoDerivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
URI: | http://hdl.handle.net/2433/278386 |
DOI(出版社版): | 10.1021/jacsau.1c00504 |
PubMed ID: | 35098239 |
出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス