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タイトル: | Geometric trade-off between contractile force and viscous drag determines the actomyosin-based motility of a cell-sized droplet |
著者: | Sakamoto, Ryota Izri, Ziane Shimamoto, Yuta Miyazaki, Makito https://orcid.org/0000-0002-4603-851X (unconfirmed) Maeda, Yusuke T. |
著者名の別形: | 坂本, 遼太 島本, 勇太 宮﨑, 牧人 前多, 裕介 |
キーワード: | ACTIN CYTOSKELETON RECONSTITUTED SYSTEMS MICROFLUIDICS CELL MIGRATION ACTIVE GELS |
発行日: | 26-Jul-2022 |
出版者: | Proceedings of the National Academy of Sciences |
誌名: | Proceedings of the National Academy of Sciences |
巻: | 119 |
号: | 30 |
論文番号: | e2121147119 |
抄録: | Cell migration in confined environments is fundamental for diverse biological processes from cancer invasion to leukocyte trafficking. The cell body is propelled by the contractile force of actomyosin networks transmitted from the cell membrane to the external substrates. However, physical determinants of actomyosin-based migration capacity in confined environments are not fully understood. Here, we develop an in vitro migratory cell model, where cytoplasmic actomyosin networks are encapsulated into droplets surrounded by a lipid monolayer membrane. We find that the droplet can move when the actomyosin networks are bound to the membrane, in which the physical interaction between the contracting actomyosin networks and the membrane generates a propulsive force. The droplet moves faster when it has a larger contact area with the substrates, while narrower confinement reduces the migration speed. By combining experimental observations and active gel theory, we propose a mechanism where the balance between sliding friction force, which is a reaction force of the contractile force, and viscous drag determines the migration speed, providing a physical basis of actomyosin-based motility in confined environments. |
記述: | 動きまわる人工細胞、その鍵は摩擦にあり --細胞が狭い空間を利用して運動する仕組みを解明--. 京都大学プレスリリース. 2022-07-21. |
著作権等: | Copyright © 2022 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). |
URI: | http://hdl.handle.net/2433/278933 |
DOI(出版社版): | 10.1073/pnas.2121147119 |
PubMed ID: | 35857875 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-07-21-2 |
出現コレクション: | 学術雑誌掲載論文等 |
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