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Title: | Isolation of the Gene Encoding Yeast Peroxisomal Isocitrate Lyase by a Combination of the Plaque Hybridization with Non-Radioactive Probes and the Amplification of Phages in a Small Scale |
Authors: | UEDA, Mitsuyoshi ![]() ![]() HIKIDA, Masaki ATOMI, Haruyuki ![]() ![]() ![]() TANAKA, Atsuo |
Issue Date: | 30-Jan-1990 |
Publisher: | Faculty of Engineering, Kyoto University |
Journal title: | Memoirs of the Faculty of Engineering, Kyoto University |
Volume: | 52 |
Issue: | 1 |
Start page: | 15 |
End page: | 24 |
Abstract: | A genomic DNA encoding isocitrate lyase, one of peroxisomal enzymes, was successfully isolated from an n-alkane-utilizable yeast genomic library prepared in a λEMBL phage by a combination of the plaque hybridization with a non-radioactive, biotin labeled, cDNA and the amplification of the phages in a small scale. Three clones, partially overlapping, with sizes of about 11, 13 and 16 kbp respectively were finally obtained. The genomic Southern blot analysis using the biotin-labeled probe suggested the presence of one isocitrate lyase gene in the genomic DNA. |
URI: | http://hdl.handle.net/2433/281409 |
Appears in Collections: | Vol.52 Part 1 |

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