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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| mfeku_52_1_15.pdf | 1.59 MB | Adobe PDF | 見る/開く |
| タイトル: | Isolation of the Gene Encoding Yeast Peroxisomal Isocitrate Lyase by a Combination of the Plaque Hybridization with Non-Radioactive Probes and the Amplification of Phages in a Small Scale |
| 著者: | UEDA, Mitsuyoshi   HIKIDA, Masaki ATOMI, Haruyuki  https://orcid.org/0000-0001-9687-6426 (unconfirmed)TANAKA, Atsuo |
| 発行日: | 30-Jan-1990 |
| 出版者: | Faculty of Engineering, Kyoto University |
| 誌名: | Memoirs of the Faculty of Engineering, Kyoto University |
| 巻: | 52 |
| 号: | 1 |
| 開始ページ: | 15 |
| 終了ページ: | 24 |
| 抄録: | A genomic DNA encoding isocitrate lyase, one of peroxisomal enzymes, was successfully isolated from an n-alkane-utilizable yeast genomic library prepared in a λEMBL phage by a combination of the plaque hybridization with a non-radioactive, biotin labeled, cDNA and the amplification of the phages in a small scale. Three clones, partially overlapping, with sizes of about 11, 13 and 16 kbp respectively were finally obtained. The genomic Southern blot analysis using the biotin-labeled probe suggested the presence of one isocitrate lyase gene in the genomic DNA. |
| URI: | http://hdl.handle.net/2433/281409 |
| 出現コレクション: | Vol.52 Part 1 |
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