Downloads: 38

Files in This Item:
File Description SizeFormat 
mfeku_52_1_15.pdf1.59 MBAdobe PDFView/Open
Title: Isolation of the Gene Encoding Yeast Peroxisomal Isocitrate Lyase by a Combination of the Plaque Hybridization with Non-Radioactive Probes and the Amplification of Phages in a Small Scale
Authors: UEDA, Mitsuyoshi  kyouindb  KAKEN_id
HIKIDA, Masaki
ATOMI, Haruyuki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-9687-6426 (unconfirmed)
TANAKA, Atsuo
Issue Date: 30-Jan-1990
Publisher: Faculty of Engineering, Kyoto University
Journal title: Memoirs of the Faculty of Engineering, Kyoto University
Volume: 52
Issue: 1
Start page: 15
End page: 24
Abstract: A genomic DNA encoding isocitrate lyase, one of peroxisomal enzymes, was successfully isolated from an n-alkane-utilizable yeast genomic library prepared in a λEMBL phage by a combination of the plaque hybridization with a non-radioactive, biotin labeled, cDNA and the amplification of the phages in a small scale. Three clones, partially overlapping, with sizes of about 11, 13 and 16 kbp respectively were finally obtained. The genomic Southern blot analysis using the biotin-labeled probe suggested the presence of one isocitrate lyase gene in the genomic DNA.
URI: http://hdl.handle.net/2433/281409
Appears in Collections:Vol.52 Part 1

Show full item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.