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タイトル: | Nano–bio interaction between human immunoglobulin G and nontoxic, near-infrared emitting water-borne silicon quantum dot micelles |
著者: | Chinnathambi, Shanmugavel https://orcid.org/0000-0001-9550-5367 (unconfirmed) Shirahata, Naoto Kumar, Mahima Karthikeyan, Subramani Abe, Katsuhiko Thangavel, Vaijayanthi Pandian, Ganesh N. |
著者名の別形: | 安倍, 克彦 |
発行日: | 2023 |
出版者: | Royal Society of Chemistry (RSC) |
誌名: | RSC Advances |
巻: | 13 |
号: | 9 |
開始ページ: | 6051 |
終了ページ: | 6064 |
抄録: | In recent years, the field of nanomaterials has exponentially expanded with versatile biological applications. However, one of the roadblocks to their clinical translation is the critical knowledge gap about how the nanomaterials interact with the biological microenvironment (nano–bio interactions). When nanomaterials are used as drug carriers or contrast agents for biological imaging, the nano–bio interaction-mediated protein conformational changes and misfolding could lead to disease-related molecular alterations and/or cell death. Here, we studied the conformation changes of human immunoglobulin G (IgG) upon interaction with silicon quantum dots functionalized with 1-decene, Pluronic-F127 (SiQD-De/F127 micelles) using UV-visible, fluorescence steady state and excited state kinetics, circular dichroism, and molecular modeling. Decene monolayer terminated SiQDs are accumulated inside the Pluronic F127 shells to form SiQD-De/F127 micelles and were shown to bind strongly with IgG. In addition, biological evaluation studies in cell lines (HeLa, Fibroblast) and medaka fish (eggs and larvae) showed enhanced uptake and minimal cytotoxicity. Our results substantiate that engineered QDs obviating the protein conformational changes could have adept bioefficacy. |
著作権等: | © 2023 The Author(s). Published by the Royal Society of Chemistry This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
URI: | http://hdl.handle.net/2433/281857 |
DOI(出版社版): | 10.1039/d3ra00552f |
PubMed ID: | 36814879 |
出現コレクション: | 学術雑誌掲載論文等 |
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