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Title: Cryo-electron microscopic structure of the nucleoprotein-RNA complex of the European filovirus, Lloviu virus
Authors: Hu, Shangfan
Fujita-Fujiharu, Yoko
Sugita, Yukihiko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6861-4840 (unconfirmed)
Wendt, Lisa
Muramoto, Yukiko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-8706-3113 (unconfirmed)
Nakano, Masahiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-4247-9008 (unconfirmed)
Hoenen, Thomas
Noda, Takeshi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-0658-4663 (unconfirmed)
Author's alias: 胡, 上帆
藤田, 陽子
杉田, 征彦
村本, 裕紀子
中野, 雅博
野田, 岳志
Keywords: Filovirus
Lloviu virus
nucleocapsid
cryo-EM
Issue Date: Apr-2023
Publisher: Oxford University Press (OUP)
National Academy of Sciences
Journal title: PNAS Nexus
Volume: 2
Issue: 4
Thesis number: pgad120
Abstract: Lloviu virus (LLOV) is a novel filovirus detected in Schreiber’s bats in Europe. The isolation of the infectious LLOV from bats has raised public health concerns. However, the virological and molecular characteristics of LLOV remain largely unknown. The nucleoprotein (NP) of LLOV encapsidates the viral genomic RNA to form a helical NP-RNA complex, which acts as a scaffold for nucleocapsid formation and de novo viral RNA synthesis. In this study, using single-particle cryo-electron microscopy, we determined two structures of the LLOV NP-RNA helical complex, comprising a full-length and a C-terminally truncated NP. The two helical structures were identical, demonstrating that the N-terminal region determines the helical arrangement of the NP. The LLOV NP-RNA protomers displayed a structure similar to that in the Ebola and Marburg virus, but the spatial arrangements in the helix differed. Structure-based mutational analysis identified amino acids involved in the helical assembly and viral RNA synthesis. These structures advance our understanding of the filovirus nucleocapsid formation, and provide a structural basis for the development of anti-filoviral therapeutics.
Description: ヨーロッパに分布するエボラウイルス近縁ウイルスの増殖機構を解明 --広範囲の抗フィロウイルス療法の開発に期待--. 京都大学プレスリリース. 2023-04-10.
Rights: © The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence, which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited.
URI: http://hdl.handle.net/2433/281873
DOI(Published Version): 10.1093/pnasnexus/pgad120
PubMed ID: 37124400
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2023-04-10-0
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