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タイトル: | An ACE2, SARS-CoV-2 spike protein binding protein, -like enzyme isolated from food-related microorganisms |
著者: | Laily, Izza Nur Takeuchi, Michiki Mizutani, Taku Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) |
著者名の別形: | 竹内, 道樹 水谷, 拓 小川, 順 |
キーワード: | Biochemistry and Molecular Biology |
発行日: | Jun-2023 |
出版者: | Oxford University Press (OUP) |
誌名: | Bioscience, Biotechnology, and Biochemistry |
巻: | 87 |
号: | 6 |
開始ページ: | 638 |
終了ページ: | 645 |
抄録: | Angiotensin-converting enzyme 2 (ACE2) is a binding target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. An ACE2-like enzyme, such as bacterial M32-carboxypeptidase (M32-CAP), is assumed to be a potential therapeutic candidate for coronavirus disease 2019 (COVID-19). Here, we screened bacteria with an ACE2-like enzyme activity from Japanese fermented food and dietary products using the fluorogenic substrate for rapid screening. The strain showing the highest activity, Enterobacter sp. 200527-13, produced an enzyme with the same hydrolytic activity as ACE2 on Angiotensin II (Ang II). The enzymatic analysis using the heterologously-expressed enzyme in Escherichia coli revealed that the enzyme catalyzes the same reaction with that of ACE2, Ang II hydrolysis to Ang 1-7, and phenylalanine. The gene sequence information showed that the enzyme belongs to the M32-CAP family. These results suggested that the selected enzyme, M32-CAP (EntCP), from Enterobacter sp. 200527-13 was identified as an ACE2-like enzyme. |
著作権等: | This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience, Biotechnology, and Biochemistry following peer review. The version of record [Izza Nur Laily and others, An ACE2, SARS-CoV-2 spike protein binding protein, -like enzyme isolated from food-related microorganisms, Bioscience, Biotechnology, and Biochemistry, Volume 87, Issue 6, June 2023, Pages 638–645] is available online at: https://doi.org/10.1093/bbb/zbad037. The full-text file will be made open to the public on 30 March 2024 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/282894 |
DOI(出版社版): | 10.1093/bbb/zbad037 |
PubMed ID: | 36997336 |
出現コレクション: | 学術雑誌掲載論文等 |
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