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タイトル: | Virological characterization of the 2022 outbreak-causing monkeypox virus using human keratinocytes and colon organoids |
著者: | Watanabe, Yukio Kimura, Izumi Hashimoto, Rina Sakamoto, Ayaka Yasuhara, Naoko Yamamoto, Takuya https://orcid.org/0000-0002-0022-3947 (unconfirmed) Genotype to Phenotype Japan (GP-Japan) Consortium Sato, Kei Takayama, Kazuo https://orcid.org/0000-0002-1132-2457 (unconfirmed) |
著者名の別形: | 渡邉, 幸夫 木村, 出海 橋本, 里菜 坂本, 綾香 山本, 拓也 佐藤, 佳 高山, 和雄 |
キーワード: | 2022 MPXV (clade IIb) colon organoids Congo Basin clade (clade I) keratinocytes monkeypox virus West African clade (clade IIa) |
発行日: | Jun-2023 |
出版者: | Wiley |
誌名: | Journal of Medical Virology |
巻: | 95 |
号: | 6 |
論文番号: | e28827 |
抄録: | The outbreak-causing monkeypox virus of 2022 (2022 MPXV) is classified as a clade IIb strain and phylogenetically distinct from prior endemic MPXV strains (clades I or IIa), suggesting that its virological properties may also differ. Here, we used human keratinocytes and induced pluripotent stem cell-derived colon organoids to examine the efficiency of viral growth in these cells and the MPXV infection-mediated host responses. MPXV replication was much more productive in keratinocytes than in colon organoids. We observed that MPXV infections, regardless of strain, caused cellular dysfunction and mitochondrial damage in keratinocytes. Notably, a significant increase in the expression of hypoxia-related genes was observed specifically in 2022 MPXV-infected keratinocytes. Our comparison of virological features between 2022 MPXV and prior endemic MPXV strains revealed signaling pathways potentially involved with the cellular damages caused by MPXV infections and highlights host vulnerabilities that could be utilized as protective therapeutic strategies against human mpox in the future. |
記述: | ヒトiPS細胞由来大腸オルガノイドおよびヒトケラチノサイトを用いたmpoxウイルス2022年株の解析. 京都大学プレスリリース. 2023-06-07. |
著作権等: | © 2023 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
URI: | http://hdl.handle.net/2433/283266 |
DOI(出版社版): | 10.1002/jmv.28827 |
PubMed ID: | 37278443 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/230607-100000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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