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pjab.98.027.pdf | 6.24 MB | Adobe PDF | 見る/開く |
タイトル: | Bacteria with a mouth: Discovery and new insights into cell surface structure and macromolecule transport |
著者: | Murata, Kousaku Kawai, Shigeyuki Hashimoto, Wataru ![]() ![]() ![]() |
著者名の別形: | 村田, 幸作 橋本, 渉 |
キーワード: | Sphingomonas alginate cell surface structure mouth-like pit flagellin ABC transporter |
発行日: | 9-Dec-2022 |
出版者: | Japan Academy |
誌名: | Proceedings of the Japan Academy, Series B |
巻: | 98 |
号: | 10 |
開始ページ: | 529 |
終了ページ: | 552 |
抄録: | A bacterium with a "mouth"-like pit structure isolated for the first time in the history of microbiology was a Gram-negative rod, containing glycosphingolipids in the cell envelope, and named Sphingomonas sp. strain A1. The pit was dynamic, with repetitive opening and closing during growth on alginate, and directly included alginate concentrated around the pit, particularly by flagellins, an alginate-binding protein localized on the cell surface. Alginate incorporated into the periplasm was subsequently transferred to the cytoplasm by cooperative interactions of periplasmic solute-binding proteins and an ATP-binding cassette transporter in the cytoplasmic membrane. The mechanisms of assembly, functions, and interactions between the above-mentioned molecules were clarified using structural biology. The pit was transplanted into other strains of sphingomonads, and the pitted recombinant cells were effectively applied to the production of bioethanol, bioremediation for dioxin removal, and other tasks. Studies of the function of the pit shed light on the biological significance of cell surface structures and macromolecule transport in bacteria. |
著作権等: | © 2022 The Author(s). Published under the terms of the CC BY-NC license |
URI: | http://hdl.handle.net/2433/283427 |
DOI(出版社版): | 10.2183/pjab.98.027 |
PubMed ID: | 36504195 |
出現コレクション: | 学術雑誌掲載論文等 |
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