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j.stemcr.2023.06.006.pdf | 5.88 MB | Adobe PDF | 見る/開く |
タイトル: | Am80, a retinoic acid receptor agonist, activates the cardiomyocyte cell cycle and enhances engraftment in the heart |
著者: | Kasamoto, Manabu Funakoshi, Shunsuke Hatani, Takeshi Okubo, Chikako Nishi, Yohei Tsujisaka, Yuta Nishikawa, Misato Narita, Megumi Ohta, Akira Kimura, Takeshi Yoshida, Yoshinori https://orcid.org/0000-0001-5511-9090 (unconfirmed) |
著者名の別形: | 笠本, 学 舟越, 俊介 羽溪, 健 大久保, 周子 西, 洋平 辻坂, 勇太 西川, 美里 成田, 恵 太田, 章 木村, 剛 吉田, 善紀 |
キーワード: | FUCCI Cell cycle Cardiac cell transplantation human induced pluripotent stem cells cardiomyocyte differentiation retinoic acid high throughput screening |
発行日: | Aug-2023 |
出版者: | Elsevier BV |
誌名: | Stem Cell Reports |
巻: | 18 |
号: | 8 |
開始ページ: | 1672 |
終了ページ: | 1685 |
抄録: | Human induced pluripotent stem cell-derived (hiPSC) cardiomyocytes are a promising source for regenerative therapy. To realize this therapy, however, their engraftment potential after their injection into the host heart should be improved. Here, we established an efficient method to analyze the cell cycle activity of hiPSC cardiomyocytes using a fluorescence ubiquitination-based cell cycle indicator (FUCCI) system. In vitro high-throughput screening using FUCCI identified a retinoic acid receptor (RAR) agonist, Am80, as an effective cell cycle activator in hiPSC cardiomyocytes. The transplantation of hiPSC cardiomyocytes treated with Am80 before the injection significantly enhanced the engraftment in damaged mouse heart for 6 months. Finally, we revealed that the activation of endogenous Wnt pathways through both RARA and RARB underlies the Am80-mediated cell cycle activation. Collectively, this study highlights an efficient method to activate cell cycle in hiPSC cardiomyocytes by Am80 as a means to increase the graft size after cell transplantation into a damaged heart. |
記述: | ヒトiPS細胞由来心筋細胞の生着能改善に向けた新しい方法. 京都大学プレスリリース. 2023-07-14. Am80, a retinoic acid receptor agonist, activates cell cycle in induced cardiomyocytes and enhances heart tissue engraftment. 京都大学プレスリリース. 2023-07-14. |
著作権等: | © 2023 The Authors. This is an open access article under the CC BY-NC-ND license. |
URI: | http://hdl.handle.net/2433/284628 |
DOI(出版社版): | 10.1016/j.stemcr.2023.06.006 |
PubMed ID: | 37451261 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/230714-100000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/230714-100000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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