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1462-2920.16427.pdf | 2.02 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Nakazawa, Takehito | en |
dc.contributor.author | Yamaguchi, Iori | en |
dc.contributor.author | Zhang, Yufan | en |
dc.contributor.author | Saka, Chinami | en |
dc.contributor.author | Wu, Hongli | en |
dc.contributor.author | Kayama, Keita | en |
dc.contributor.author | Kawauchi, Moriyuki | en |
dc.contributor.author | Sakamoto, Masahiro | en |
dc.contributor.author | Honda, Yoichi | en |
dc.contributor.alternative | 中沢, 威人 | ja |
dc.contributor.alternative | 坂, 知奈美 | ja |
dc.contributor.alternative | 香山, 渓太 | ja |
dc.contributor.alternative | 河内, 護之 | ja |
dc.contributor.alternative | 坂本, 正弘 | ja |
dc.contributor.alternative | 本田, 与一 | ja |
dc.date.accessioned | 2023-11-13T08:17:42Z | - |
dc.date.available | 2023-11-13T08:17:42Z | - |
dc.date.issued | 2023-10 | - |
dc.identifier.uri | http://hdl.handle.net/2433/286021 | - |
dc.description.abstract | Lignin-modifying enzymes (LMEs), which include laccases (Lacs), manganese peroxidases (MnPs), versatile peroxidases (VPs), and lignin peroxidases (LiPs), have been considered key factors in lignin degradation by white-rot fungi because they oxidize lignin model compounds and depolymerize synthetic lignin in vitro. However, it remains unclear whether these enzymes are essential/important in the actual degradation of natural lignin in plant cell walls. To address this long-standing issue, we examined the lignin-degrading abilities of multiple mnp/vp/lac mutants of Pleurotus ostreatus. One vp2/vp3/mnp3/mnp6 quadruple-gene mutant was generated from a monokaryotic wild-type strain PC9 using plasmid-based CRISPR/Cas9. Also, two vp2/vp3/mnp2/mnp3/mnp6, two vp2/vp3/mnp3/mnp6/lac2 quintuple-gene mutants, and two vp2/vp3/mnp2/mnp3/mnp6/lac2 sextuple-gene mutants were generated. The lignin-degrading abilities of the sextuple and vp2/vp3/mnp2/mnp3/mnp6 quintuple-gene mutants on the Beech wood sawdust medium reduced drastically, but not so much for those of the vp2/vp3/mnp3/mnp6/lac2 mutants and the quadruple mutant strain. The sextuple-gene mutants also barely degraded lignin in Japanese Cedar wood sawdust and milled rice straw. Thus, this study presented evidence that the LMEs, especially MnPs and VPs, play a crucial role in the degradation of natural lignin by P. ostreatus for the first time. | en |
dc.language.iso | eng | - |
dc.publisher | Wiley | en |
dc.rights | © 2023 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd. | en |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.title | Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9 | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Environmental Microbiology | en |
dc.identifier.volume | 25 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 1909 | - |
dc.identifier.epage | 1924 | - |
dc.relation.doi | 10.1111/1462-2920.16427 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 37218079 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 19H03017 | - |
datacite.awardNumber | 19F19095 | - |
datacite.awardNumber | 21K18224 | - |
datacite.awardNumber | 19K22332 | - |
datacite.awardNumber | 18KK0178 | - |
datacite.awardNumber | 18H02254 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03017/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19F19095/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K18224/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22332/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18KK0178/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02254/ | - |
dc.identifier.pissn | 1462-2912 | - |
dc.identifier.eissn | 1462-2920 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
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jpcoar.awardTitle | 木材腐朽菌のタンパク質分泌機構の強化と新規機能の付与 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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