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Title: Derivation of the small-angle scattering profile of a target biomacromolecule from a profile deteriorated by aggregates. AUC–SAS
Authors: Morishima, Ken
Inoue, Rintaro  kyouindb  KAKEN_id
Sugiyama, Masaaki
Author's alias: 守島, 健
井上, 倫太郎
杉山, 正明
Keywords: small-angle X-ray scattering
small-angle neutron scattering
analytical ultracentrifugation
protein solutions
aggregates
biomacromolecules
Issue Date: 1-Jun-2023
Publisher: International Union of Crystallography (IUCr)
Journal title: Journal of Applied Crystallography
Volume: 56
Issue: 3
Start page: 624
End page: 632
Abstract: Aggregates cause a fatal problem in the structural analysis of a biomacromolecule in solution using small-angle X-ray or neutron scattering (SAS): they deteriorate the scattering profile of the target molecule and lead to an incorrect structure. Recently, an integrated method of analytical ultracentrifugation (AUC) and SAS, abbreviated AUC–SAS, was developed as a new approach to overcome this problem. However, the original version of AUC–SAS does not offer a correct scattering profile of the target molecule when the weight fraction of aggregates is higher than ca 10%. In this study, the obstacle point in the original AUC–SAS approach is identified. The improved AUC–SAS method is then applicable to a solution with a relatively larger weight fraction of aggregates (≤20%).
Rights: Published under a CC BY 4.0 licence
URI: http://hdl.handle.net/2433/286121
DOI(Published Version): 10.1107/s1600576723002406
PubMed ID: 37284265
Appears in Collections:Journal Articles

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