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Title: Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules
Authors: Komatsu, Shodai
Ohno, Hirohisa  kyouindb  KAKEN_id
Saito, Hirohide  kyouindb  KAKEN_id
Author's alias: 小松, 将大
大野, 博久
齊藤, 博英
Keywords: Genetic engineering
Synthetic biology
Issue Date: 17-Nov-2023
Publisher: Springer Nature
Journal title: Nature Communications
Volume: 14
Thesis number: 7256
Abstract: Controlling gene expression in response to specific molecules is an essential technique for regulating cellular functions. However, current platforms with transcription and translation regulators have a limited number of detectable molecules to induce gene expression. Here to address these issues, we present a Target-dependent RNA polymerase (TdRNAP) that can induce RNA transcription in response to the intracellular target specifically recognized by single antibody. By substituting the fused antibody, we demonstrate that TdRNAPs respond to a wide variety of molecules, including peptides, proteins, RNA, and small molecules, and produce desired transcripts in human cells. Furthermore, we show that multiple TdRNAPs can construct orthogonal and multilayer genetic circuits. Finally, we apply TdRNAP to achieve cell-specific genome editing that is autonomously triggered by detecting the target gene product. TdRNAP can expand the molecular variety for controlling gene expression and provide the genetic toolbox for bioengineering and future therapeutic applications.
Description: 抗体を用いた標的依存性RNAポリメラーゼの開発 多様な細胞内分子に応答する遺伝子発現制御のプラットフォーム. 京都大学プレスリリース. 2023-11-17.
Using Single-antibodies as a New Ingenious Tool to Build Bio-circuitry. 京都大学プレスリリース. 2023-11-17.
Rights: © The Author(s) 2023
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
DOI(Published Version): 10.1038/s41467-023-42802-5
PubMed ID: 37978180
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