ダウンロード数: 39
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.celrep.2023.113431.pdf | 6.05 MB | Adobe PDF | 見る/開く |
タイトル: | Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD |
著者: | Mae, Shin-Ichi Hattanda, Fumihiko Morita, Hiroyoshi Nozaki, Aya Katagiri, Naoko Ogawa, Hanako Teranaka, Kaori Nishimura, Yu Kudoh, Aoi Yamanaka, Sanae Matsuse, Kyoko Ryosaka, Makoto Watanabe, Akira Soga, Tomoyoshi Nishio, Saori Osafune, Kenji https://orcid.org/0000-0001-7238-2763 (unconfirmed) |
著者名の別形: | 前, 伸一 八反田, 文彦 盛田, 大義 野崎, 彩 片桐, 直子 寺中, 香 西村, 優 工藤, 葵 山中, 早苗 松瀬, 恭子 兩阪, 誠 渡辺, 亮 曽我, 朋義 西尾, 妙織 長船, 健二 |
キーワード: | human induced pluripotent stem cell autosomal dominant polycystic kidney disease ureteric bud collecting duct organoid in vitro model high-throughput screening |
発行日: | 26-Dec-2023 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 42 |
号: | 12 |
論文番号: | 113431 |
抄録: | In autosomal dominant polycystic kidney disease (ADPKD), renal cyst lesions predominantly arise from collecting ducts (CDs). However, relevant CD cyst models using human cells are lacking. Although previous reports have generated in vitro renal tubule cyst models from human induced pluripotent stem cells (hiPSCs), therapeutic drug candidates for ADPKD have not been identified. Here, by establishing expansion cultures of hiPSC-derived ureteric bud tip cells, an embryonic precursor that gives rise to CDs, we succeed in advancing the developmental stage of CD organoids and show that all CD organoids derived from PKD1−/− hiPSCs spontaneously develop multiple cysts, clarifying the initiation mechanisms of cystogenesis. Moreover, we identify retinoic acid receptor (RAR) agonists as candidate drugs that suppress in vitro cystogenesis and confirm the therapeutic effects on an ADPKD mouse model in vivo. Therefore, our in vitro CD cyst model contributes to understanding disease mechanisms and drug discovery for ADPKD. |
記述: | 腎集合管オルガノイドを用いた多発性嚢胞腎モデルの作製 iPS創薬により治療薬候補を発見、治験開始へ. 京都大学プレスリリース. 2023-12-01. Developing more advanced renal organoids to model polycystic kidney disease. 京都大学プレスリリース. 2023-12-01. |
著作権等: | © 2023 The Authors. This is an open access article under the CC BY license. |
URI: | http://hdl.handle.net/2433/286477 |
DOI(出版社版): | 10.1016/j.celrep.2023.113431 |
PubMed ID: | 38039961 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/231201-010000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/231201-010000.html |
出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス