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dc.contributor.author | Yamashiro, Sawako | en |
dc.contributor.author | Rutkowski, David M. | en |
dc.contributor.author | Lynch, Kelli Ann | en |
dc.contributor.author | Liu, Ying | en |
dc.contributor.author | Vavylonis, Dimitrios | en |
dc.contributor.author | Watanabe, Naoki | en |
dc.contributor.alternative | 山城, 佐和子 | ja |
dc.contributor.alternative | 劉, 穎 | ja |
dc.contributor.alternative | 渡邊, 直樹 | ja |
dc.date.accessioned | 2024-01-15T08:13:02Z | - |
dc.date.available | 2024-01-15T08:13:02Z | - |
dc.date.issued | 2023-12-20 | - |
dc.identifier.uri | http://hdl.handle.net/2433/286625 | - |
dc.description | メカニカル・アンフォールディングが細胞内流動力を伝達する --走る電車の力を伝えるにはゴム人間が役に立つ--. 京都大学プレスリリース. 2024-01-12. | ja |
dc.description.abstract | Force transmission at integrin-based adhesions is important for cell migration and mechanosensing. Talin is an essential focal adhesion (FA) protein that links F-actin to integrins. F-actin constantly moves on FAs, yet how Talin simultaneously maintains the connection to F-actin and transmits forces to integrins remains unclear. Here we show a critical role of dynamic Talin unfolding in force transmission. Using single-molecule speckle microscopy, we found that the majority of Talin are bound only to either F-actin or the substrate, whereas 4.1% of Talin is linked to both structures via elastic transient clutch. By reconstituting Talin knockdown cells with Talin chimeric mutants, in which the Talin rod subdomains are replaced with the stretchable β-spectrin repeats, we show that the stretchable property is critical for force transmission. Simulations suggest that unfolding of the Talin rod subdomains increases in the linkage duration and work at FAs. This study elucidates a force transmission mechanism, in which stochastic molecular stretching bridges two cellular structures moving at different speeds. | en |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | © The Author(s) 2023 | en |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Actin | en |
dc.subject | Focal adhesion | en |
dc.subject | Single-molecule biophysics | en |
dc.title | Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Nature Communications | en |
dc.identifier.volume | 14 | - |
dc.relation.doi | 10.1038/s41467-023-44018-z | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 8468 | - |
dc.address | Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies; Department of Pharmacology, Kyoto University Graduate School of Medicine | en |
dc.address | Department of Physics, Lehigh University | en |
dc.address | Department of Physics, Lehigh University; University of South Florida | en |
dc.address | Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies | en |
dc.address | Department of Physics, Lehigh University | en |
dc.address | Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies; Department of Pharmacology, Kyoto University Graduate School of Medicine | en |
dc.identifier.pmid | 38123541 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2024-01-12-2 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 21K06150 | - |
datacite.awardNumber | 22H04843 | - |
datacite.awardNumber | 21H05780 | - |
datacite.awardNumber | 22H00456 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K06150/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-22H04843/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-21H05780/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H00456/ | - |
dc.identifier.eissn | 2041-1723 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 高精度単分子イメージングによるアクチンダイナミクス-接着斑連関の生理的意義の解明 | ja |
jpcoar.awardTitle | 高解像度分子可視化による力学的方向情報伝達機構の解明 | ja |
jpcoar.awardTitle | 蛍光単分子イメージングによる微小環境感知メカニズムの可視化解明 | ja |
jpcoar.awardTitle | 分子・構造変換のリアルタイム可視化からの病気の解明と治療戦略 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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