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タイトル: | Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner |
著者: | Hamad, Nesreen Yoneda, Ryoma So, Masatomo Kurokawa, Riki Nagata, Takashi https://orcid.org/0000-0002-3733-2709 (unconfirmed) Katahira, Masato https://orcid.org/0000-0003-0336-7660 (unconfirmed) |
著者名の別形: | 永田, 崇 片平, 正人 |
キーワード: | Intrinsically disordered proteins Long non-coding RNAs Neurological disorders Protein aggregation |
発行日: | 4-May-2021 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 11 |
論文番号: | 9523 |
抄録: | Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation state as an instant response to the shear stress caused by usual pipetting even at a low FUS concentration, 100 nM. It was revealed that non-coding RNA can suppress the transformation of FUS into aggregates. The suppressive effect of RNA on FUS aggregation is sequence-dependent. These results suggested that the non-coding RNA could be a prospective suppressor of FUS aggregation caused by mechanistic stress in cells. Our finding might pave the way for more research on the role of RNAs as aggregation inhibitors, which could facilitate the development of therapies for neurodegenerative diseases. |
著作権等: | © The Author(s) 2021 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/286725 |
DOI(出版社版): | 10.1038/s41598-021-89075-w |
PubMed ID: | 33947944 |
出現コレクション: | 学術雑誌掲載論文等 |
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