このアイテムのアクセス数: 67
このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| j.xpro.2023.102555.pdf | 3.55 MB | Adobe PDF | 見る/開く |
| タイトル: | Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain |
| 著者: | Mino, Takeharu Nonaka, Hiroshi    https://orcid.org/0000-0003-4623-2926 (unconfirmed)Sakamoto, Seiji Oh, Jae Hoon Hamachi, Itaru https://orcid.org/0000-0002-3327-3916 (unconfirmed) |
| 著者名の別形: | 野中, 洋 美野, 丈晴 坂本, 清志 浜地, 格 |
| キーワード: | Neuroscience Molecular/Chemical Probes Chemistry |
| 発行日: | 15-Sep-2023 |
| 出版者: | Elsevier BV |
| 誌名: | STAR Protocols |
| 巻: | 4 |
| 号: | 3 |
| 論文番号: | 102555 |
| 抄録: | Here, we present fixation-driven chemical crosslinking of exogenous ligands, a protocol to visualize the distribution of exogenously administered small molecules in the mouse brain. We first describe the probe design of the small molecules of interest and the probe microinjection into a live mouse brain in detail. We then detail procedures for paraformaldehyde-perfusion fixation. This approach is especially useful for imaging-based evaluation of the small-molecule ligands distribution in mouse brain tissue relying on their interaction with endogenous proteins. |
| 著作権等: | © 2023 This is an open access article under the CC BY-NC-ND license. |
| URI: | http://hdl.handle.net/2433/286873 |
| DOI(出版社版): | 10.1016/j.xpro.2023.102555 |
| PubMed ID: | 37660299 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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