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タイトル: | FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid–liquid phase separation |
著者: | Kaizuka, Takeshi Hirouchi, Taisei ![]() ![]() ![]() Saneyoshi, Takeo ![]() Shirafuji, Toshihiko Collins, Mark O. Grant, Seth G. N. Hayashi, Yasunori ![]() ![]() ![]() Takumi, Toru |
著者名の別形: | 貝塚, 剛志 廣内, 大成 實吉, 岳郎 白藤, 俊彦 林, 康紀 内匠, 透 |
キーワード: | Neurons Hippocampus Neuronal dendrites Immunoprecipitation Proteomes Condensation Transfection Dendritic structure |
発行日: | Mar-2024 |
出版者: | Public Library of Science (PLoS) |
誌名: | PLOS Biology |
巻: | 22 |
号: | 3 |
論文番号: | e3002006 |
抄録: | Proteome analyses of the postsynaptic density (PSD), a proteinaceous specialization beneath the postsynaptic membrane of excitatory synapses, have identified several thousands of proteins. While proteins with predictable functions have been well studied, functionally uncharacterized proteins are mostly overlooked. In this study, we conducted a comprehensive meta-analysis of 35 PSD proteome datasets, encompassing a total of 5, 869 proteins. Employing a ranking methodology, we identified 97 proteins that remain inadequately characterized. From this selection, we focused our detailed analysis on the highest-ranked protein, FAM81A. FAM81A interacts with PSD proteins, including PSD-95, SynGAP, and NMDA receptors, and promotes liquid–liquid phase separation of those proteins in cultured cells or in vitro. Down-regulation of FAM81A in cultured neurons causes a decrease in the size of PSD-95 puncta and the frequency of neuronal firing. Our findings suggest that FAM81A plays a crucial role in facilitating the interaction and assembly of proteins within the PSD, and its presence is important for maintaining normal synaptic function. Additionally, our methodology underscores the necessity for further characterization of numerous synaptic proteins that still lack comprehensive understanding. |
記述: | 神経シナプス後部のタンパク質の集積を司る因子を発見. 京都大学プレスリリース. 2024-03-08. |
著作権等: | © 2024 Kaizuka et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/287296 |
DOI(出版社版): | 10.1371/journal.pbio.3002006 |
PubMed ID: | 38452102 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2024-03-08-1 |
出現コレクション: | 学術雑誌掲載論文等 |
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