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このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| dnogk02623.pdf | Dissertation_全文 | 15.26 MB | Adobe PDF | 見る/開く |
| ynogk02623.pdf | Abstract_要旨 | 543.73 kB | Adobe PDF | 見る/開く |
| タイトル: | Studies on enzymes and reaction conditions in recombinase polymerase amplification |
| その他のタイトル: | リコンビナーゼポリメラーゼ増幅法の酵素と反応条件に関する研究 |
| 著者: | Kevin, Maafu Juma |
| キーワード: | Isothermal DNA amplification Recombinase polymerase amplification Single stranded-DNA binding protein Hexahistidine tag micro temperature gradient gel electrophoresis |
| 発行日: | 25-Mar-2024 |
| 出版者: | Kyoto University |
| 学位授与大学: | 京都大学 |
| 学位の種類: | 新制・課程博士 |
| 取得分野: | 博士(農学) |
| 報告番号: | 甲第25357号 |
| 学位記番号: | 農博第2623号 |
| 学位授与年月日: | 2024-03-25 |
| 研究科・専攻: | 京都大学大学院農学研究科食品生物科学専攻 |
| 論文調査委員: | (主査)教授 保川 清, 教授 井上 和生, 教授 谷 史人 |
| 学位授与の要件: | 学位規則第4条第1項該当 |
| 著作権等: | 1. "Comparison of sensitivity and rapidness of PCR, recombinase polymerase amplification, and RNA-specific amplification for detection of Rice yellow mottle virus" Kevin Maafu Juma, Kenji Kojima, Teisuke Takita, Keiko T. Natsuaki, Kiyoshi Yasukawa ("Journal of Biological Macromolecules" January 2021, Volume 21, Issue 1, Pages 27-32) doi: 10.14533/jbm.21.27 The final publication is available at J-STAGE via https://doi.org/10.14533/jbm.21.27 2. "Solvent engineering studies on recombinase polymerase amplification" Kenji Kojima, Kevin Maafu Juma, Shihomi Akagi, Kaichi Hayashi, Teisuke Takita, Ciara K. O'Sullivan, Shinsuke Fujiwara, Yukiko Nakura, Itaru Yanagihara, Kiyoshi Yasukawa ("Journal of Bioscience and Bioengineering" February 2021, Volume 131, Issue 2, Pages 219-224) doi: 10.1016/j.jbiosc.2020.10.001 The final publication is available at Elsevier via https://doi.org/10.1016/j.jbiosc.2020.10.001 3. "Optimization of reaction condition of recombinase polymerase amplification to detect SARS-CoV-2 DNA and RNA using a statistical method" Kevin Maafu Juma, Teisuke Takita, Kenji Ito, Masaya Yamagata, Shihomi Akagi, Emi Arikawa, Kenji Kojima, Manish Biyani, Shinsuke Fujiwara, Yukiko Nakura, Itaru Yanagihara, Kiyoshi Yasukawa ("Biochemical and Biophysical Research Communications" August 2021, Volume 567, Pages 195-200) doi: 10.1016/j.bbrc.2021.06.023 The final publication is available at Elsevier via https://doi.org/10.1016/j.bbrc.2021.06.023 4. "Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA" Kevin Maafu Juma, Teisuke Takita, Masaya Yamagata, Mika Ishitani, Kaichi Hayashi, Kenji Kojima, Koichiro Suzuki, Yuri Ando, Wakao Fukuda, Shinsuke Fujiwara, Yukiko Nakura, Itaru Yanagihara, Kiyoshi Yasukawa ("Molecular Biology Reports" January 2022, Volume 49, Issue 4, Pages 2847?2856) doi: 10.1007/s11033-021-07098-y The final publication is available at Springer link via https://doi.org/10.1007/s11033-021-07098-y 5. "Recombinase polymerase amplification using novel thermostable strand-displacing DNA polymerases from Aeribacillus pallidus and Geobacillus zalihae" Kevin Maafu Juma, Eisuke Inoue, Kengo Asada, Wakao Fukuda, Kenta Morimoto, Masaya Yamagata, Teisuke Takita, Kenji Kojima, Koichiro Suzuki, Yukiko Nakura, Itaru Yanagihara, Shinsuke Fujiwara, Kiyoshi Yasukawa ("Journal of Bioscience and Bioengineering" April 2023, Volume 135, Issue 4, Pages 282-290) doi: 10.1016/j.jbiosc.2023.01.009 The final publication is available at Elsevier via https://doi.org/10.1016/j.jbiosc.2023.01.009 6. "Detection of SARS-CoV-2 spike protein D614G mutation using ?TGGE" Kevin Maafu Juma, Kenta Morimoto, Vishnu Sharma, Kirti Sharma, Radhika Biyani, Manish Biyani, Teisuke Takita, Kiyoshi Yasukawa ("Molecular Biology Reports") in press |
| DOI: | 10.14989/doctor.k25357 |
| URI: | http://hdl.handle.net/2433/288791 |
| 出現コレクション: | 100 博士(農学) |
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