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タイトル: Defective flow space limits the scaling up of turbulence bioreactors for platelet generation
著者: Okamoto, Haruki
Fujio, Kosuke
Nakamura, Sou
Harada, Yasuo
Hayashi, Hideki
Higashi, Natsumi
Ninomiya, Atsushi
Tanaka, Ryota
Sugimoto, Naoshi
Takayama, Naoya
Kaneda, Atsushi
Sawaguchi, Akira
Kato, Yoshikazu
Eto, Koji
著者名の別形: 岡本, 陽己
藤尾, 康祐
中村, 壮
原田, 康男
東, 奈津美
二宮, 敦
田中, 亮多
杉本, 直志
澤口, 朗
江藤, 浩之
キーワード: Biologics
Regenerative medicine
発行日: 17-Jun-2024
出版者: Springer Nature
誌名: Communications Engineering
巻: 3
論文番号: 77
抄録: To complement donor-dependent platelets supplies, we previously developed an ex vivo manufacturing system using induced pluripotent stem cell (iPSC)-derived expandable immortalized megakaryocyte progenitor cell lines (imMKCLs), and a turbulent flow bioreactor to generate iPSC-derived platelets products (iPSC-PLTs). However, the tank size of the bioreactor was limited to 10 L. Here we examined the feasibility of scaling up to 50 L with reciprocal motion by two impellers. Under optimized turbulence parameters corresponding to 10 L bioreactor, 50 L bioreactor elicited iPSC-PLTs with intact in vivo hemostatic function but with less production efficiency. This insufficiency was caused by increased defective turbulent flow space. A computer simulation proposed that designing 50 L turbulent flow bioreactor with three impellers or a new bioreactor with a modified rotating impeller and unique structure reduces this space. These findings indicate that large-scale iPSC-PLTs manufacturing from cultured imMKCLs requires optimization of the tank structure in addition to optimal turbulent energy and shear stress.
記述: 血小板製造の規模拡大を実現する新たな培養装置の検討と設計. 京都大学プレスリリース. 2024-06-17.
著作権等: © The Author(s) 2024
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
URI: http://hdl.handle.net/2433/290225
DOI(出版社版): 10.1038/s44172-024-00219-y
関連リンク: https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/240617-180000.html
出現コレクション:学術雑誌掲載論文等

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