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dc.contributor.authorSchiphof, Kimen
dc.contributor.authorKawauchi, Moriyukien
dc.contributor.authorTsuji, Kenyaen
dc.contributor.authorYoshimi, Akiraen
dc.contributor.authorTanaka, Chihiroen
dc.contributor.authorNakazawa, Takehitoen
dc.contributor.authorHonda, Yoichien
dc.contributor.alternative河内, 護之ja
dc.contributor.alternative辻, 健也ja
dc.contributor.alternative吉見, 啓ja
dc.contributor.alternative田中, 千尋ja
dc.contributor.alternative中沢, 威人ja
dc.contributor.alternative本田, 与一ja
dc.date.accessioned2024-11-11T02:46:42Z-
dc.date.available2024-11-11T02:46:42Z-
dc.date.issued2024-06-
dc.identifier.urihttp://hdl.handle.net/2433/290238-
dc.description.abstractChitin is an essential structural component of fungal cell walls composed of transmembrane proteins called chitin synthases (CHSs), which have a large range of reported effects in ascomycetes; however, are poorly understood in agaricomycetes. In this study, evolutionary and molecular genetic analyses of chs genes were conducted using genomic information from nine ascomycete and six basidiomycete species. The results support the existence of seven previously classified chs clades and the discovery of three novel basidiomycete-specific clades (BI–BIII). The agaricomycete fungus Pleurotus ostreatus was observed to have nine putative chs genes, four of which were basidiomycete-specific. Three of these basidiomycete specific genes were disrupted in the P. ostreatus 20b strain (ku80 disruptant) through homologous recombination and transformants were obtained (Δchsb2, Δchsb3, and Δchsb4). Despite numerous transformations Δchsb1 was unobtainable, suggesting disruption of this gene causes a crucial negative effect in P. ostreatus. Disruption of these chsb2–4 genes caused sparser mycelia with rougher surfaces and shorter aerial hyphae. They also caused increased sensitivity to cell wall and membrane stress, thinner cell walls, and overexpression of other chitin and glucan synthases. These genes have distinct roles in the structural formation of aerial hyphae and cell walls, which are important for understanding basidiomycete evolution in filamentous fungi.en
dc.language.isoeng-
dc.publisherElsevier BVen
dc.rights© 2024 The Author(s). Published by Elsevier Inc.en
dc.rightsThis is an open access article under the CC BY-NC license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/-
dc.subjectChitinen
dc.subjectAgaricomyceteen
dc.subjectBasidiomycete specific chitin synthaseen
dc.subjectCell wallen
dc.subjectPleurotus ostreatusen
dc.titleFunctional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatusen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleFungal Genetics and Biologyen
dc.identifier.volume172-
dc.relation.doi10.1016/j.fgb.2024.103893-
dc.textversionpublisher-
dc.identifier.artnum103893-
dc.identifier.pmid38657898-
dcterms.accessRightsopen access-
dc.identifier.eissn1087-1845-
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