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Title: | Molecular identification of hyaluronate lyase, not hyaluronidase, as an intrinsic hyaluronan-degrading enzyme in Clostridium perfringens strain ATCC 13124 |
Authors: | Kumon, Tomoya Oiki, Sayoko Hashimoto, Wataru |
Author's alias: | 久門, 知也 老木, 紗予子 橋本, 渉 |
Keywords: | Bacteriology Enzymes Glycobiology Pathogens |
Issue Date: | 22-Oct-2024 |
Publisher: | Springer Nature |
Journal title: | Scientific Reports |
Volume: | 14 |
Thesis number: | 24266 |
Abstract: | Clostridium perfringens, an opportunistic pathogen, produces mu-toxin hyaluronidases including endo-β-N-acetylglucosaminidases (Nags) as a virulence invasion factor. To clarify an intrinsic factor for degradation of host extracellular hyaluronan, we focused on hyaluronate lyase (HysA), distinct from endo-β-N-acetylglucosaminidases. C. perfringens strain ATCC 13124 was found to assimilate host-derived extracellular mucosubstances, hyaluronan and mucin, which induced expression of the hyaluronan-related genetic cluster, including hyaluronate lyase gene (hysA), but repressed endo-β-N-acetylglucosaminidase genes. This genetic cluster is conserved in some strains of C. perfringens. The recombinant strain ATCC 13124 hyaluronate lyase HysA showed an hyaluronan-degrading activity through β-elimination reaction. The hyaluronan-degrading enzyme in the culture supernatant of strain ATCC 13124 exhibited the lyase activity and was identical to the recombinant hyaluronate lyase on the native-PAGE gel, followed by activity straining. These results demonstrated that the intrinsic hyaluronan-degrading enzyme of C. perfringens strain ATCC 13124 is hyaluronate lyase HysA, but not hyaluronidases NagH, NagJ, and NagK. |
Description: | 日和見病原性ウェルシュ菌のヒアルロン酸分解機構 --抗菌剤開発への展開 --. 京都大学プレスリリース. 2024-10-23. |
Rights: | © The Author(s) 2024 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holde. |
URI: | http://hdl.handle.net/2433/290625 |
DOI(Published Version): | 10.1038/s41598-024-73955-y |
PubMed ID: | 39438475 |
Related Link: | https://www.kyoto-u.ac.jp/ja/research-news/2024-10-23 |
Appears in Collections: | Journal Articles |
This item is licensed under a Creative Commons License