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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-024-73696-y.pdf | 3.08 MB | Adobe PDF | 見る/開く |
| タイトル: | Structural characterization of green fluorescent protein in the I-state. |
| 著者: | Takeda, Ryota Tsutsumi, Erika Okatsu, Kei    https://orcid.org/0000-0001-8949-3750 (unconfirmed)Fukai, Shuya https://orcid.org/0000-0002-1241-1443 (unconfirmed)Takeda, Kazuki https://orcid.org/0000-0002-4094-6816 (unconfirmed) |
| 著者名の別形: | 武田, 遼太 堤, 恵理華 尾勝, 圭 深井, 周也 竹田, 一旗 |
| キーワード: | Fluorescent protein Hydrogen bond Intermediate Non-covalent interaction QM/MM X-ray crystallography |
| 発行日: | 1-Oct-2024 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 14 |
| 論文番号: | 22832 |
| 抄録: | Green fluorescent protein (GFP) is widely utilized as a fluorescent tag in biochemical fields. Whereas the intermediate (I) state has been proposed in the photoreaction cycle in addition to the A and B states, until now the structure of I has only been estimated by computational studies. In this paper, we report the crystal structures of the I stabilizing variants of GFP at high resolutions where respective atoms can be observed separately. Comparison with the structures in the other states highlights the structural feature of the I state. The side chain of one of the substituted residues, Val203, adopts the gauche- conformation observed for Thr203 in the A state, which is different from the B state. On the other hand, His148 interacts with the chromophore by ordinary hydrogen bonding with a distance of 2.85 Å, while the weaker interaction by longer distances is observed in the A state. Therefore, it was indicated that it is possible to distinguish three states A, B and I by the two hydrogen bond distances Oγ-Thr203···Oη-chromophore and Nδ1-His148···Oη-chromophore. We discuss the characteristics of the I intermediate of wild-type GFP on the bases of the structure estimated from the variant structures by quantum chemical calculations. |
| 著作権等: | © The Author(s) 2024 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
| URI: | http://hdl.handle.net/2433/290912 |
| DOI(出版社版): | 10.1038/s41598-024-73696-y |
| PubMed ID: | 39353998 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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