このアイテムのアクセス数: 27
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
acschembio.4c00625.pdf | 7.11 MB | Adobe PDF | 見る/開く |
タイトル: | Development of a Versatile Plant-Derived Mitochondrial Targeting Sequence Based on a Reporter Protein Sorting Analysis and Biological Information |
著者: | Abe, Naoya Odahara, Masaki Morey, Shamitha Rao Numata, Keiji ![]() ![]() ![]() |
キーワード: | Mitochondria Monomers Peptides and proteins Plants Targeting |
発行日: | 20-Dec-2024 |
出版者: | American Chemical Society (ACS) |
誌名: | ACS chemical biology |
巻: | 19 |
号: | 12 |
開始ページ: | 2515 |
終了ページ: | 2524 |
抄録: | Methods for the delivery of exogenous substances to specific organelles are important because each organelle functions according to its own role. Specifically, mitochondria play an important role in energy production. Recently, plant mitochondrial transformation via delivery methods to mitochondria has been actively researched. Mitochondrial targeting sequences (MTSs) are essential for transporting bioactive molecules, such as nucleic acids, to mitochondria. However, the selectivity and efficacy of MTSs as carrier molecules in plants are not yet sufficient. In this study, we developed an effective MTS in plants via a quantitative comparison of the targeting functions of several MTSs. The presequence of HSP60 from Nicotiana tabacum, which is highly similar to that of several other model plants, showed high mitochondrial-targeting ability among the MTSs tested. This result suggests the applicability of the HSP60 presequence for MTSs in various plants. We further investigated this HSP60 presequence through stepwise shortening on the basis of secondary structure prediction, aiming to simplify synthesis and increase the solubility of the peptides. As shown by assessment of the mitochondrial targeting ability, the 15 residues from the N-terminus of the HSP60 presequence for the MTS, which is particularly conserved among various model plants, retained a targeting efficacy comparable to that of the full-length HSP60 presequence. This developed sequence from the HSP60 sequence is a promising MTS for transfection into plant mitochondria. |
著作権等: | Copyright © 2024 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY-NC-ND 4.0 |
URI: | http://hdl.handle.net/2433/292344 |
DOI(出版社版): | 10.1021/acschembio.4c00625 |
PubMed ID: | 39622491 |
出現コレクション: | 学術雑誌掲載論文等 |

このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス