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lsa.202402837.pdf | 2.44 MB | Adobe PDF | View/Open |
Title: | Human iPS cell–derived respiratory organoids as a model for respiratory syncytial virus infection |
Authors: | Hashimoto, Rina Watanabe, Yukio Keshta, Abeer Sugiyama, Masaya Kitai, Yuki Hirabayashi, Ai Yasuhara, Naoko Morimoto, Shiho Sakamoto, Ayaka Matsumura, Yasufumi Nishimura, Hidekazu Noda, Takeshi Yamamoto, Takuya Nagao, Miki ![]() ![]() ![]() Takeda, Makoto Takayama, Kazuo |
Issue Date: | Jul-2025 |
Publisher: | Life Science Alliance, LLC |
Journal title: | Life science alliance |
Volume: | 8 |
Issue: | 7 |
Abstract: | Respiratory syncytial virus (RSV) is a seasonal respiratory pathogen that primarily affects young children, potentially causing severe lower respiratory tract disease. Despite the high disease burden, understanding of RSV pathophysiology remains limited. To address this, advanced RSV infection models are needed. Whereas HEp-2 cells are widely used because of their high susceptibility to RSV, they do not accurately reflect the host response of the human respiratory tract. In this study, we evaluated human-induced pluripotent stem cell-derived respiratory organoids, which contain respiratory epithelial cells, immune cells, fibroblasts, and vascular endothelial cells, for their potential to model RSV infection and support pharmaceutical research. RSV-infected organoids exhibited high viral genome and protein expression, epithelial layer destruction, and increased collagen accumulation. Pro-inflammatory cytokine levels in culture supernatants also increased post-infection. Furthermore, RSV infection was significantly inhibited by monoclonal antibodies (nirsevimab, palivizumab, suptavumab, or clesrovimab), although ribavirin showed limited efficacy. These findings highlight the utility of respiratory organoids for RSV research. |
Rights: | This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
URI: | http://hdl.handle.net/2433/294607 |
DOI(Published Version): | 10.26508/lsa.202402837 |
PubMed ID: | 40262853 |
Appears in Collections: | Journal Articles |

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