Pressure inactivation of enzyme : some kinetic aspects of pressure inactivation of chymotrypsin

Abstract

The inactivation of chymotrypsin by pressure has been investigated kinetically including the effect of pH, concentration of enzyme, magnitude of pressure, repeated compression. Inactivation process of chymotrypsin by high pressure is of the first order kinetic. The temperature coefficient of the rate of inactivation is positive, and thermodynamic functions in the activation process of inactivation are as follows; ΔH≠>0, ΔF≠>0, ΔS≠<0 and ΔV≒<0. From the experimental results, it is found that the process of the inactivation of chymotrypsin by high pressure is almost similar to that of trypsin.