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Title: Pleiotrophic functions of Epstein-Barr virus nuclear antigen-1 (EBNA-1) and oriP differentially contribute to the efficiency of transfection/expression of exogenous gene in mammalian cells
Authors: Kishida, Tsunao
Asada, Hidetsugu  kyouindb  KAKEN_id  orcid (unconfirmed)
Kubo, Koji
Sato, Yuko T.
Shin-Ya, Masaharu
Imanishi, Jiro
Yoshikawa, Kenichi
Mazda, Osam
Author's alias: 吉川, 研一
Keywords: EBNA1
Artificial chromosome
Issue Date: Jan-2008
Publisher: Elsevier
Citation: Kishida, T., H. Asada, K. Kubo, Y. Sato, M. Shin-Ya,J. Imanishi, K.Yoshikawa & O. Mazda, “Pleiotrophic Functions of Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1) and oriP Differentially Contribute to the Efficacy of Transfection/expression of Exogenous Gene in Mammalian Cells” Journal of Biotechnology. 133(2), 201-207. (2008).
Journal title: Journal of Biotechnology
Volume: 133
Issue: 2
Start page: 201
End page: 207
Abstract: The EBNA1 gene and oriP sequence, originally derived from the EBV genome, provide plasmid vectors with artificial chromosome (AC)-like characteristics, including cytoplasm-to-nuclear transport, nuclear retention, replication and segregation of the DNA, while transcriptional up-regulation has been suggested as another activity of the EBNA1/oriP. Transfection as well as expression rates of various nonviral delivery vehicles are highly improved by inserting these genetic elements into plasmid DNA constructs. Here we differentially analyzed the contribution of each function of the EBNA1/oriP to the efficacy of electroporation-mediated genetic delivery and expression in mammalian cells. It was found that the EBNA1/oriP-mediated acceleration of genetic delivery and expression was predominantly due to the promotion of cytoplasm-to-nuclear recruitment as well as enhancement of transcription, while the episomal replication of the EBV-AC was not essentially involved.
Rights: Copyright © 2008 Elsevier B.V. All rights reserved.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.jbiotec.2007.08.035
PubMed ID: 17935815
Appears in Collections:Journal Articles

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