このアイテムのアクセス数: 306
| タイトル: | A Simple Method for Measurement of Mitochondrial DNA Content in Total Cell DNA Using Non-Radioactive DNA Probes |
| 著者: | 浅野, 仁子  中村, 正彦 浅野, 朗 |
| 著者名の別形: | Asano, Kimiko Nakamura, Masahiko Asano, Akira |
| 発行日: | 1991 |
| 出版者: | 京都大学医療技術短期大学部 |
| 誌名: | 京都大学医療技術短期大学部紀要 |
| 巻: | 11 |
| 開始ページ: | 27 |
| 終了ページ: | 37 |
| 抄録: | Quantitative measurement of mitochondrial DNA (mtDNA) has been very troublesome, because its content in total DNA was very low and quantitative separation of the mtDNA from nuclear DNA (nucDNA) was almost impossible. By using the technique for cloning cells carrying the mtDNA sequences, specific detection of mtDNA became possible in the presence of large excess of nucDNA by DNA-DNA hybridization. For this purpose, we prepared mtDNA probes labeled with a non-radioactive substrate, which made the procedure easier and increased possibility of application. This method revealed that the contents of mtDNA in rat liver strikingly decreased with aging. |
| URI: | http://hdl.handle.net/2433/49357 |
| 出現コレクション: | 第11号 |
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