Part I. Studies on crude materials (Studies on Anti-tuberculous Low Molecular Factors in Various Organs of Rabbits)

Abstract

1. The tuberculostatic activity of low molecular substances (able to pass through a cellophane membrane) in various organ extracts, (muscle, liver, kidney, brain, lung and spleen) of rabbits was investigated. And it was confirmed that substances which can inhibit in vitro growth of the H37Rv strain of tubercle bacilli were actually present. The activity of these substances is, in descending order, muscle (strongest), liver, kidney and brain, lung and spleen. 2. These substances are soluble in water and methanol but insoluble in ethanol, ethyl ether and acetone. 3. These substances are stable to heating at 120℃ for 30min. 4. The activity of these substances is not increased by immunization of the rabbits.